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Inflammation and Cancer

Methods and Protocols: Volume 2, Molecular Analysis and Pathways

  • Book
  • © 2009

Overview

Editors:
  1. Serguei V. Kozlov

    1. National Cancer Institute at Frederick, SAIC-Frederick, Inc., Frederick, USA

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  • Contains key readily reproducible experimental approaches certain to be useful as technologies continue to develop
  • Details basic requirements for the most recent techniques still awaiting experimental substantiation
  • Fosters rigorous scientific interactions among basic and clinical researchers aimed at further molecular demarcation of the elaborate pathways leading from inflammation to tumor formation
  • Describes in-depth pathway analysis, instrumental to the construction and navigation through molecular charts for cellular processes from gametogenesis through cell senescence
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 512)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Crossroads of Inflammation and Cancer: Molecular Aspects

    1. Immunohistochemical Analysis of 8-Nitroguanine, A Nitrative DNA Lesion, in Relation to Inflammation-Associated Carcinogenesis

      • Yusuke Hiraku, Shosuke Kawanishi
      Pages 3-14

    2. Molecular Analysis of Genetic Instability Caused by Chronic Inflammation

      • Bin Yan, Yuanlin Peng, Chuan-Yuan Li
      Pages 15-28

    3. Obesity Provides a Permissive Milieu in Inflammation-Associated Carcinogenesis: Analysis of Insulin and IGF Pathways

      • Nomeli P. Nunez, Stephen D. Hursting, Shoshana Yakar, Dan Fowler, Charles Vinson
      Pages 29-37

    4. Detecting and Modulating the NF-kB Activity in Human Immune Cells: Generation of Human Cell Lines with Altered Levels of NF-κB

      • Fui G. Goh, Helen Banks, Irina A. Udalova
      Pages 39-54

    5. Inflammation-Related Aberrant Patterns of DNA Methylation: Detection and Role in Epigenetic Deregulation of Cancer Cell Transcriptome

      • Hiromu Suzuki, Minoru Toyota, Yutaka Kondo, Yasuhisa Shinomura
      Pages 55-69

    6. cDNA Microarray Analysis of Inflammatory Breast Cancer Signatures

      • Steven J. Van Laere, Peter B. Vermeulen, Luc Y. Dirix
      Pages 71-98

    7. Analysis of the TLR/NF-κB Pathway in Antigen-Presenting Cells in Malignancies Promoted by Inflammation

      • J. Magarian Blander
      Pages 99-117

    8. Induced Nitric Oxide Synthase as a Major Player in the Oncogenic Transformation of Inflamed Tissue

      • Guang-Yu Yang, Sofia Taboada, Jie Liao
      Pages 119-156

  3. Molecular and Cellular Approaches to Diagnostics and Drug Target Discovery In Inflammation-Related Oncogenesis

    1. Development of a Cell-Based Assay to Quantify the Inflammatory Potential of Test Substances and Screen Compound Libraries for Anti-cancer Drug Candidates in a High-Throughput Format

      • Serguei V. Kozlov
      Pages 159-167

    2. The NF-κB Transcription Factor Pathway as a Therapeutic Target in Cancer: Methods for Detection of NF-κB Activity

      • Claudio Mauro, Francesca Zazzeroni, Salvatore Papa, Concetta Bubici, Guido Franzoso
      Pages 169-207

    3. Inhibiting Proinflammatory NF-κB Signaling Using Cell-Penetrating NEMO Binding Domain Peptides

      • Lisa A. Madge, Michael J. May
      Pages 209-232

    4. Quantitative Measurement of Cytokine Expression in Synoviocytes Derived from Rheumatoid Arthritis Patients

      • Scott Thomson, Irina A. Udalova
      Pages 233-248

    5. Molecular Characterization of Inflammation-Induced JNK/c-Jun Signaling Pathway in Connection with Tumorigenesis

      • Bozena Kaminska
      Pages 249-264

    6. Molecular Characterization of STAT Signaling in Inflammation and Tumorigenesis

      • Alicja Adach, Aleksandra Ellert-Miklaszewska, Bozena Kaminska
      Pages 265-278

    7. Inflammation and Tumor Progression: A Lesson from TNF-α-Dependent FAK Signaling in Cholangiocarcinoma

      • Naing Naing Mon, Toshio Kokuryo, Michinari Hamaguchi
      Pages 279-293

    8. Cyclooxygenase Inhibitors Induce Colon Cancer Cell Apoptosis Via PPARδ → 14-3-3ε Pathway

      • Kenneth K. Wu, Jun-Yang Liou
      Pages 295-307

    9. PPARβ/δ Agonist Increases the Expression of PGE2 Receptor Subtype EP4 in Human Lung Carcinoma Cells

      • Jeffrey D. Ritzenthaler, Jesse Roman, ShouWei Han
      Pages 309-323

    10. Investigating Macrophage and Malignant Cell Interactions In Vitro

      • Thorsten Hagemann, Toby Lawrence
      Pages 325-332

    11. Cellular Magnetic Resonance Imaging Using Superparamagnetic Anionic Iron Oxide Nanoparticles: Applications to In Vivo Trafficking of Lymphocytes and Cell-Based Anticancer Therapy

      • Pierre Smirnov
      Pages 333-353
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Keywords

About this book

According to the most recent clinical oncology data, one out of seven newly diagnosed malignancies worldwide result from infection and chronic inflammation in conjunction with cancer. In Inflammation and Cancer: Methods and Protocols, expert researchers deliver a systematic guide to techniques addressing various aspects of experimental cancer biology, selectively focused on inflammation-mediated tumorigenesis, while promoting improvisations on a per-case basis. Volume 2, Molecular Analysis and Pathways is devoted to an extensive description of experimental strategies aimed at investigating the molecular cross-talks between components of cell signaling chains and their ramifications in diagnostic development and drug target discovery. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step laboratory protocols, and a notes section, which examines tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Inflammation and Cancer: Methods and Protocols promises to serve as a vital guide and resource for investigators and clinicians working toward the goal of combatting the estimated 2, 200 inflammation-related oncogenesis fatalities occurring every day.

Reviews

From the reviews: “Chapters in this volume are designed to be guides to techniques addressing various aspects of experimental cancer biology. … this volume will earn a place on the lab benches of investigators and clinicians who are studying inflammation-related oncogenesis. … this book can provide a source of information for establishing new techniques as well as suggestions for investigating particular aspects of tumorigenesis.” (Marion C. Cohen, Doody’s Review Service, December, 2009)

Editors and Affiliations

  • National Cancer Institute at Frederick, SAIC-Frederick, Inc., Frederick, USA

    Serguei V. Kozlov

Bibliographic Information

  • Book Title: Inflammation and Cancer

  • Book Subtitle: Methods and Protocols: Volume 2, Molecular Analysis and Pathways

  • Editors: Serguei V. Kozlov

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-530-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60327-529-3Published: 26 May 2009

  • Softcover ISBN: 978-1-61737-905-5Published: 19 November 2010

  • eBook ISBN: 978-1-60327-530-9Published: 05 April 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 408

  • Number of Illustrations: 51 b/w illustrations

  • Topics: Oncology, Cancer Research, Laboratory Medicine

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