Overview
- Fully updated from the first edition to offer cutting-edge methods for SNP detection and analysis
- Offers a unique overview of next generation sequencing and genotyping approaches
- Contains review chapters comparing methods capabilities, error rates, and costs
- Provides advanced techniques as well as descriptions of simple approaches which do not require sophisticated equipment
- Low and high-throughput, single and multiplex applications are presented
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 578)
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Table of contents (28 protocols)
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Front Matter
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Introduction to Single Nucleotide Polymorphisms
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Front Matter
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Bioinformatic Analysis of SNPs
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SNP Identification and Detection Strategies: Whole Genome Sequencing and Re-Sequencing
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Front Matter
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Prescreening (Melting Based) Methods for SNP Discovery and Analysis
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Front Matter
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Prescreening (Conformation Based) Methods for SNP Discovery and Analysis
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Front Matter
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Keywords
About this book
In recent years, single nucleotide polymorphisms have received increased and special attention in a rapidly developing field of personalized medicine and drug treatment. Comprising more than eighty percent of all known polymorphisms, single nucleotide polymorphisms (SNPs) are primarily responsible for phenotypic differences between individuals, and have been suggested to affect the development of diseases in humans and the response to drug treatment and environmental stress. In Single Nucleotide Polymorphisms: Methods and Protocols, Second Edition, expert researchers explore the latest advances in this area, highlighting the substantial progress that has been made in SNP genotyping, examining recent developments in high-throughput genotyping approaches, and exploring our new understanding of the impact of SNPs on gene function. Chapters address the impact of SNPs on phenotype, examine SNP databases, look at methods that have been applied for SNP bioinformatics discovery and analysis, and discuss advanced experimental approaches used for SNP detection. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Current and innovative, Single Nucleotide Polymorphisms: Methods and Protocols, Second Edition is an essential guidebook for individual researchers as well as institutions and companies working in the field.
Reviews
From the reviews of the second edition:
“This second edition brings 11 new chapters … . Its target audience continues to be researchers and laboratory personnel. One helpful feature the book includes is a set of ‘notes’ at the end of each chapter … to be very useful for readers choosing to utilize a technique for the first time. … Overall, this book is a good addition to any laboratory setting actively working on complex human traits or interested in personalized medicine and drug treatments.” (Alexandre R. Vieira, Birth Defects Research (Part A): Clinical and Molecular Teratology, Vol. 88 (12), 2010)Editors and Affiliations
Bibliographic Information
Book Title: Single Nucleotide Polymorphisms
Book Subtitle: Methods and Protocols
Editors: Anton A. Komar
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-411-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-410-4Published: 27 October 2009
Softcover ISBN: 978-1-61779-679-1Published: 25 February 2012
eBook ISBN: 978-1-60327-411-1Published: 19 September 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 464
Number of Illustrations: 82 b/w illustrations, 18 illustrations in colour
Additional Information: Originally published as volume 212 in the series: Methods in Molecular Biology
Topics: Human Genetics, Cell Biology, Gene Function