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Peptide Microarrays

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Marina Cretich

    1. Inst. Chimica del Riconoscimento, CNR, Milano, Italy

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  2. Marcella Chiari

    1. Inst. Chimica del Riconoscimento, CNR, Milano, Italy

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    You can also search for this editor in PubMed   Google Scholar

  • Provides an easily accessible reference volume for peptide microarrays experiments written by leading researchers in the field
  • Details the process of turning biological observations into a high-throughput screening methodology
  • Covers a wide range of peptide microarrays formats and protocols
  • Presents a modular approach to experimental design, screening modalities, detection technologies, and approaches to data analysis
  • Comprehensive guide to developing, running, and analyzing peptide microarrays

Part of the book series: Methods in Molecular Biology (MIMB, volume 570)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Exploring and Profiling Protein Function with Peptide Arrays

      • Victor E. Tapia, Bernhard Ay, Rudolf Volkmer
      Pages 3-17

    3. Peptide Arrays for Enzyme Profiling

      • Alexandra Thiele, Johannes Zerweck, Mike Schutkowski
      Pages 19-65

    4. Using Peptide Array to Identify Binding Motifs and Interaction Networks for Modular Domains

      • Shawn S.-C. Li, Chenggang Wu
      Pages 67-76

    5. Molecular Simulations of Peptides: A Useful Tool for the Development of New Drugs and for the Study of Molecular Recognition

      • Massimiliano Meli, Giorgio Colombo
      Pages 77-153

  3. Applications

    1. Front Matter

      Pages 155-155
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    2. Synthesis of Peptide Arrays Using SPOT-Technology and the CelluSpots-Method

      • Dirk F.H. Winkler, Kai Hilpert, Ole Brandt, Robert E.W. Hancock
      Pages 157-174

    3. Rapid Identification of Linear Protein Domain Binding Motifs Using Peptide SPOT Arrays

      • Douglas J. Briant, James M. Murphy, Genie C. Leung, Frank Sicheri
      Pages 175-185

    4. Characterization of Kinase Target Phosphorylation Consensus Motifs Using Peptide SPOT Arrays

      • Genie C. Leung, James M. Murphy, Doug Briant, Frank Sicheri
      Pages 187-195

    5. CelluSpots™: A Reproducible Means of Making Peptide Arrays for the Determination of SH2 Domain Binding Specificity

      • Chenggang Wu, Shawn S.-C. Li
      Pages 197-202

    6. High-Density Peptide Microarrays for Reliable Identification of Phosphorylation Sites and Upstream Kinases

      • Alexandra Thiele, Johannes Zerweck, Matthias Weiwad, Gunter Fischer, Mike Schutkowski
      Pages 203-219

    7. Epitope Mapping of Human Chromogranin A by Peptide Microarrays

      • Marina Cretich, Renato Longhi, Angelo Corti, Francesco Damin, Gabriele Di Carlo, Valentina Sedini et al.
      Pages 221-232

    8. Antimicrobial Peptide Arrays for Detection of Inactivated Biothreat Agents

      • Chris R. Taitt, Stella H. North, Nadezhda V. Kulagina
      Pages 233-255

    9. Mapping Functional Prion–Prion Protein Interaction Sites Using Prion Protein Based Peptide-Arrays

      • Alan Rigter, Jan Priem, Drophatie Timmers-Parohi, Jan Pm Langeveld, Alex Bossers
      Pages 257-271

    10. A Designed Peptide Chip: Protein Fingerprinting Technology with a Dry Peptide Array and Statistical Data Mining

      • Kenji Usui, Kin-ya Tomizaki, Hisakazu Mihara
      Pages 273-284

  4. Technological Advancements

    1. Front Matter

      Pages 285-285
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    2. Peptide Microarrays on Bisphenol A Polycarbonate

      • Vianney Souplet, Clément Roux, Oleg Melnyk
      Pages 287-297

    3. Self-Assembly of PNA-Encoded Peptides into Microarrays

      • Francois Debaene, Nicolas Winssinger
      Pages 299-307

    4. A Novel Combinatorial Approach to High-Density Peptide Arrays

      • Mario Beyer, Ines Block, Kai König, Alexander Nesterov, Simon Fernandez, Thomas Felgenhauer et al.
      Pages 309-316
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Keywords

About this book

Due to their versatility, along with the diminishing costs of library synthesis and the growth of commercial support, peptide microarrays will likely expand beyond being just a research tool into an adaptable and powerful platform to be harnessed for wider drug discovery and point-of-care applications. In Peptide Microarrays: Methods and Protocols, experts in the field provide a cutting-edge view of peptide array technology, its applications, and technical issues. After examining how peptides can characterize proteins and clarify, at the amino acid level, the molecular recognition events in which they are involved, the volume goes on to cover topics such as the production and use of peptide arrays for enzyme and binding motifs characterization, epitope mapping, and diagnostics, the newest technological advancements, as well as software and web tools for the design of peptide arrays and for the analysis of output data. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Peptide Microarrays: Methods and Protocols promises to serve scientists with its unique insights and novel solutions in peptide array technology in order to advance this vital field.

Editors and Affiliations

  • Inst. Chimica del Riconoscimento, CNR, Milano, Italy

    Marina Cretich, Marcella Chiari

Bibliographic Information

  • Book Title: Peptide Microarrays

  • Book Subtitle: Methods and Protocols

  • Editors: Marina Cretich, Marcella Chiari

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-394-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-60327-393-0Published: 25 November 2009

  • Softcover ISBN: 978-1-61779-666-1Published: 07 March 2012

  • eBook ISBN: 978-1-60327-394-7Published: 31 July 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 434

  • Number of Illustrations: 116 b/w illustrations, 2 illustrations in colour

  • Topics: Protein Science, Biochemistry, general, Microarrays

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