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Lipoproteins and Cardiovascular Disease

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Lita A. Freeman

    1. Pulmonary & Vascular Medicine Branch, National Institutes of Health (NIH) National Heart, Lung & Blood Institute, Bethesda, USA

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  • Encompasses lipoprotein-centered methodologies designed for all experience levels
  • Features detailed protocols that are lab-ready
  • Includes expert tips and key implementation advice to ensure successful results

Part of the book series: Methods in Molecular Biology (MIMB, volume 1027)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  3. Sequencing

    1. Front Matter

      Pages 137-137
      Download chapter PDF

    2. Sequencing PCR-Amplified DNA in Lipoprotein and Cardiovascular Disease Research

      • Victoria Youngblood, James G. Taylor VI
      Pages 139-155

    3. Introduction to Next-Generation Nucleic Acid Sequencing in Cardiovascular Disease Research

      • Lena Diaw, Victoria Youngblood, James G. Taylor VI
      Pages 157-179

  4. Transgenic, Knockout, and Knockdown Methodologies

    1. Front Matter

      Pages 181-181
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    2. Strategies for Designing Transgenic DNA Constructs

      • Chengyu Liu
      Pages 183-201

    3. Purification of Plasmid and BAC Transgenic DNA Constructs

      • Chengyu Liu, Yubin Du, Wen Xie, Changyun Gui
      Pages 203-215

    4. Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production

      • Chengyu Liu, Wen Xie, Changyun Gui, Yubin Du
      Pages 217-232

    5. Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes

      • Boris L. Vaisman
      Pages 233-251

    6. Generation of General and Tissue-Specific Gene Knockout Mouse Models

      • Xian-Cheng Jiang
      Pages 253-271

    7. Adeno-associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism

      • William R. Lagor, Julie C. Johnston, Martin Lock, Luk H. Vandenberghe, Daniel J. Rader
      Pages 273-307

    8. Modulation of Lipoprotein Metabolism by Antisense Technology: Preclinical Drug Discovery Methodology

      • Rosanne M. Crooke, Mark J. Graham
      Pages 309-324

  5. Special Topics

    1. Front Matter

      Pages 325-325
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    2. Chromatin Immunoprecipitation

      • Grant D. Barish, Rajenda K. Tangirala
      Pages 327-342
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Keywords

About this book

The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies.  In Lipoproteins and Cardiovascular Disease: Methods and Protocols, experts in the field present a compendium of advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and vascular biology research, bringing together in a single volume an updated set of protocols and strategies for methods now driving the most recent advances, along with classical methods that are still widely used.  Among the many topics covered in this cutting-edge work, the book delves into crucial techniques such as quantitative real-time PCR, microarrays, RT-PCR laser capture microdissection, and tissue-specific gene overexpression, knockout, and knockdown methodologies, including AAV as a liver-directed gene delivery vehicle.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable notes which highlight tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and easy to use, Lipoproteins and Cardiovascular Disease: Methods and Protocols serves both novices and experts alike as a complete guide for any researcher with an interest in lipoproteins and their significant biological effects.

Editors and Affiliations

  • Pulmonary & Vascular Medicine Branch, National Institutes of Health (NIH) National Heart, Lung & Blood Institute, Bethesda, USA

    Lita A. Freeman

Bibliographic Information

  • Book Title: Lipoproteins and Cardiovascular Disease

  • Book Subtitle: Methods and Protocols

  • Editors: Lita A. Freeman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60327-369-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-60327-368-8Published: 05 August 2013

  • Softcover ISBN: 978-1-4939-5690-6Published: 23 August 2016

  • eBook ISBN: 978-1-60327-369-5Published: 04 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 391

  • Number of Illustrations: 27 b/w illustrations, 23 illustrations in colour

  • Topics: Protein Science, Cardiology

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