Editors:
- Comprehensive guide to developing, running, and analyzing assays to monitor protein secretion from single proteins to proteome scale
- Presents a modular approach to experimental design, covering model biological systems, screening modalities, detection technologies, and bioinformatics tools
- Covers a wide range of cell-based and in vitro assay formats and systems
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 619)
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Table of contents (26 protocols)
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Front Matter
About this book
Over the past thirty years, many elegant genetic and biochemical approaches have been combined in order to advance the study of protein secretion and the necessary navigation through cell membranes, yet, despite this progress, less than two hundred membrane protein structures are known, nowhere near the complete inventory that the discovered protein export systems suggest. In Protein Secretion: Methods and Protocols, leading experts in the field provide robust, well-established protocols to elucidate the multiplicity of tools that have been developed to study protein sorting, membrane targeting, transmembrane crossing, and secretion across multiple membranes. With examples involving both prokaryotic and eukaryotic organisms, the volume covers subjects ranging from bioinformatics and proteomics to fundamental enzymology and genetics to cell biology, structural analyses, and biophysics. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the key materials and reagents, step-by-step, readily reproducible protocols, and detailed notes on troubleshooting and avoiding known pitfalls.
Comprehensive and dependable, Protein Secretion: Methods and Protocols focuses on well-characterized paradigms so that scientists studying a vast array of subjects from biochemistry and genetics to biotechnology and biopharmaceuticals can benefit and expand upon their vital research.
Editors and Affiliations
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Technology - Hellas (FORTH), Inst. Molecular Biology & Biotechnology, Foundation for Research &, Iraklion, Greece
Anastassios Economou
Bibliographic Information
Book Title: Protein Secretion
Book Subtitle: Methods and Protocols
Editors: Anastassios Economou
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-412-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2010
Hardcover ISBN: 978-1-60327-167-7Published: 26 April 2010
Softcover ISBN: 978-1-4939-5707-1Published: 23 August 2016
eBook ISBN: 978-1-60327-412-8Published: 26 April 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 426
Topics: Enzymology, Biochemistry, general, Proteomics