Overview
- Editors:
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Brian C.-S. Liu
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Brigham and Women’s Hospital, Harvard Medical School, Boston, USA
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Joshua R. Ehrlich
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Rhode Island Sea Grant College Program, Brigham and Women’s Hospital, Boston, USA
- Provides time tested, reproducible laboratory methods and protocols authored by experts in the field, including tips and hints
- Presents the most up to date and useful proteomic techniques for profiling human tissue
- Provides indispensable protocols for biomarker discovery, therapeutic response profiling, and high throughput based techniques
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Table of contents (13 protocols)
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- Christine R. Rozanas, Stacey M. Loyland
Pages 1-18
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- Injae Shin, Alina D. Zamfir, Bin Ye
Pages 19-39
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- Audrey Gagnon, Qian Shi, Bin Ye
Pages 41-56
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- Sau-Mei Leung, Rebecca L. Pitts
Pages 57-70
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- Adrianna S. Rodriguez, Benjamin H. Espina, Virginia Espina, Lance A. Liotta
Pages 71-90
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- Stephen M. Hewitt, Robert A. Star
Pages 105-112
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- Virginia Espina, Julia Wulfkuhle, Valerie S. Calvert, Lance A. Liotta, Emanuel F. Petricoin III
Pages 113-128
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- Xin Jiang, Lesile Roth, Stephanie Han, Xianqiang Li
Pages 153-161
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- Oliver W. Tassinari, Margarita Aponte, Robert J. Caiazzo Jr., Brian C.-S. Liu
Pages 163-173
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- Joshua R. Ehrlich, Liangdan Tang, Robert J. Caiazzo Jr., Daniel W. Cramer, Shu-Kay Ng, Shu-Wing Ng et al.
Pages 175-192
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Back Matter
Pages 221-225
About this book
With the sequencing of the human genome complete, the field of proteomics, still in its early stages, has become an important and informative field of biomedical research. Many of the protocols and techniques commonly employed by proteomics researchers have been refined over the past several years, while at the same time, new and innovative methods have also been developed. Significantly, many of the protocols used in the study of the human proteome have been developed to examine proteins derived specifically from cell lines, body fluids or human tissues. As a consequence, not all protocols are compatible with the full-range of protein sources commonly used in the laboratory. The current volume on Tissue Proteomics seeks to bring together a number of useful and innovative protocols developed particularly for the proteomic profiling of human tissues. The first two protocols presented in this work focus on high-throughput g- based techniques. Chapter 1 describes 2-D DIGE, a method used to examine the entire proteome within discreet pI fractions, whereas Chapter 2 details several complementary techniques for the specific analysis of glycoproteins found in tissue samples. The next chapters, Chapters 3 and 4, focus on SELDI-MS and MALDI-TOF, two techniques that make use of mass spectrometry for the characterization and identification of proteins. These techniques may be used alone or in combination with other proteomic methods, such gel-based assays, where mass spectrometry is useful for additional characterization and identification of proteins.
Editors and Affiliations
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Brigham and Women’s Hospital, Harvard Medical School, Boston, USA
Brian C.-S. Liu
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Rhode Island Sea Grant College Program, Brigham and Women’s Hospital, Boston, USA
Joshua R. Ehrlich
