Human Embryogenesis

Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols, internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and state-of-the-art, Human Embryogenesis: Methods and Protocols provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis.

• Cloning
• Embryo
• Embryotoxicity
• Ethics and law
• Fetal and placental development
• Implantation
• In vitro fertilization
• Steroidogenesis
• ethics
• gene expression
• genes
• molecular biology
• molecular mechanisms

From the reviews:

“This book in the Methods in Molecular Biology Series covers human embryogenesis with sections on gametes, fertilization, embryo development, implantation, and organ system development. … Bench researchers in a variety of disciplines would find the research protocols useful. … This unique book for researchers covers an array of topics that are generally not found together in one volume.” (Linda R. Nelson, Doody’s Review Service, March, 2010)

“Human Embryogenesis offers useful and detailed descriptions of a variety of tissue preparation and analytic techniques. … may be helpful for investigators to determine whether some of the materials listed in the detailed protocols can be legally obtained in their jurisdiction. … This book would be useful for investigators who are moving into certain new areas of embryogenesis research, as well as post doctoral and graduate students with experimental interests closely aligned to the procedures that are included.” (Armand Lione, Birth Defects Research (Part A): Clinical and Molecular Teratology, Vol. 88, June, 2010)

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