Avidin-Biotin Interactions

1. Front Matter

   Pages i-xiii

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2. Preparation of Avidin Conjugates

   • Rosaria P. Haugland, Mahesh K. Bhalgat

   Pages 1-12

3. Coupling of Antibodies With Biotin

   • Rosaria P. Haugland, Wendy W. You

   Pages 13-23

4. Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin

   • Sara R. Zwart, Brandon J. Lewis

   Pages 25-34

5. Use of the Avidin (Imino)Biotin System as a General Approach to Affinity Precipitation

   • Ruth Freitag, Frank Hilbrig

   Pages 35-50

6. Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis

   • Bao-Shiang Lee, Sangeeth Krishnanchettiar, Syed Salman Lateef, Shalini Gupta

   Pages 51-61

7. Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS–PAGE Using Biotin-4-Fluorescein

   • Nicolas Humbert, Thomas R. Ward

   Pages 63-71

8. Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives

   • Andreas Ebner, Markus Marek, Karl Kaiser, Gerald Kada, Christoph D. Hahn, Bernd Lackner et al.

   Pages 73-88

9. Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins

   • Christoph Rösli, Jascha-N. Rybak, Dario Neri, Giuliano Elia

   Pages 89-100

10. High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli

    • Nicolas Humbert, Peter Schürmann, Andrea Zocchi, Jean-Marc Neuhaus, Thomas R. Ward

    Pages 101-110

11. Endogenous Biotin in Rat Brain

    • Bruce E. McKay, Michael L. Molineux, Ray W. Turner

    Pages 111-128

12. Pseudo-Immunolabelling With the Avidin–Biotin–Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina

    • Willem Kamphuis, Jan Klooster

    Pages 129-138

13. Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones

    • Gabriela Camporeale, Yap Ching Chew, Alice Kueh, Gautam Sarath, Janos Zempleni

    Pages 139-148

14. Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay

    • Kirsten Liebert, Albert Jeltsch

    Pages 149-155

15. Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin

    • Elisabeth D. Coene, Michael K. Shaw, David J. Vaux

    Pages 157-170

16. Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure

    • Karel Smetana Jr., Sabine André

    Pages 171-185

17. A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin

    • Krista McCutcheon

    Pages 187-207

18. Biotin–Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications

    • Donald M. Mock, Anna Bogusiewicz

    Pages 209-220

19. Back Matter

    Pages 221-232

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For researchers today, the avidin-biotin interaction is among the most widely exploited among biomedical scientists. Despite its popularity, avidin-biotin methodology suffers from a lack of clarity associated with popular, low cost, prefabricated kits used by many in the field. In Avidin-Biotin Interactions: Methods and Applications, leaders in avidin-biotin methodology share their knowledge relating to the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. Although avidin-biotin based kits based have been produced for quite some time and remain a widely used methodology, many of the chapters in this text describe situations in which the previous methodology failed due to some factor, and the authors developed new and better ways for the avidin-biotin system to be utilized. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist. Each chapter in Avidin-Biotin Interactions: Methods and Applications contains not only excellent descriptions of protocols for the lab but also the experience of researchers who used specific methodology, found a problem with that methodology in a new setting, and ultimately devised ways to minimize or obviate the limitations of the technology.

• Affinity chromatography
• Affinity electrophoresis
• Antibodies
• Antigen
• DNA
• Glucose
• Herman
• Organelle
• Polyacrylamid-Gelelektrophorese
• Protein avidin
• Vitamin biotin
• proteins

Aus den Rezensionen: “... Das ... editierte Methodenbuch gliedert sich in siebzehn Beiträge, die von auf dem jeweiligen Gebiet ausgewiesenen Autoren verfasst wurden. ... Die angegebenen Protokolle ermöglichen, einem Methodenbuch entsprechend, eine zeitsparende und kostengünstige Präparation ... Positiv ist auch die gute Darstellung der Faktoren, die für Biotinylierungsreaktionen ... kritisch sind. ... Das Buch ist nach Form und Inhalt in jedem Fall geeignet, dem Leser die weitere Vertiefung in die aktuelle Literatur zu ermöglichen und Hinweise für Applikationen zu geben. ... eine umfassende Informationsquelle und Protokolle zur Verfügung ...“ (Dr. rer. nat. H. Walzel, in: Laboratoriums-Medizin: journal of laboratory medicine, 2009, Vol.33, Issue 2, S. 129 f.)

• Mead Johnson Nutritionals, Evansville

  Robert J. McMahon

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