Editors:
- First methods collection devoted entirely to the SUMO system
- Provides comprehensive practical advice from leading experts in the field
- Covers a range of complementary approaches for SUMO target identification
- Presents step-by-step protocols for the purification and manipulation of SUMO conjugation and de-conjugation enzymes
- Includes a description of SUMO fusion technology for improved recombinant protein production
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 497)
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Table of contents (20 protocols)
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Front Matter
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Introduction
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Identification of Sumo Targets
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Identification Of Sumo Interactors
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Functional Analysis Of Sumoylation In Vivo
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Biochemical Studies Of Sumo Conjugation
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Biochemical Studies of Sumo Deconjugation
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Manipulation And Alternative Uses Of The Sumo System
About this book
Reviews
From the reviews:
"The modification of proteins by small ubiquitin-like modification (SUMO) is relevant to virtually all areas of molecular and cell biology. SUMO Protocols is a quite comprehensive volume describing various in vivo and in vitro techniques … . The target audience is likely to be postdoctoral fellows and PhD students in laboratories not only dedicated to SUMO pathways but those beginning their initial forays in the field … and regulated by SUMO. … for those pursuing SUMO-specific modification this is a worthwhile addition to their bookshelves." (Peter O’Hare, Microbiology Today, July, 2009)
Editors and Affiliations
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London Research Institute Clare Hall Laboratories, Cancer Research UK, Herts, UK
Helle D. Ulrich
Bibliographic Information
Book Title: SUMO Protocols
Editors: Helle D. Ulrich
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-566-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-934115-80-0
Softcover ISBN: 978-1-61737-945-1
eBook ISBN: 978-1-59745-566-4
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 324
Number of Illustrations: 70 b/w illustrations
Topics: Protein Science, Cell Biology, Biochemistry, general, Proteomics, Enzymology