Viral Applications of Green Fluorescent Protein

Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols, leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology™ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.

• GFP
• Promoter
• Viral gene therapy
• Viral vectors
• Virus
• gene transfer
• genes
• molecular biology

From the reviews:

"One of the most useful modifications that have been made to viruses is the ability to encode tracers such as green fluorescent protein (GFP) … thereby allowing not only study of the virus itself, but also the behaviour of infected cells. As the title indicates, it is a methods text, giving useful practical details important to those attempting to carry out similar studies." (Christopher Ring, Microbiology Today, November, 2009)

"This volume in the Methods in Molecular Biology series is a compilation of laboratory protocols organized into 24 chapters dealing with the diverse viral delivery systems for green fluorescent proteins. … The book is written primarily for practicing scientists involved in the study of green fluorescent expression in live cells and experimental animals. … provides a number of updated protocols for the practicing investigator. … a valuable companion for all researchers involved in the use of green fluorescent proteins in biological systems." (Ruben Mestril, Doody’s Review Service, September, 2009)

“This book focuses on a very important area of application of green fluorescent protein (GFP) and its variants … . The significance of viral applications of fluorescent proteins is well covered … . it will certainly be a valuable source of information and a good basis for laboratory protocols for researchers from all different fields of life sciences.” (Johannes Schmid, ChemBioChem, Vol. 10, 2009)

“Cover the use of Green Fluorescent Protein (GFP) technology to study aspects of the replication of viruses and the majority of the chapters describe the use of viruses and GFP as research tools in a much wider context. … I enjoyed the book for the breadth of topics and snapshots of research … . a more likely home for this book is a university or institute library where its eclectic contents will have a sufficiently wide audience.” (Paul Monaghan, Infocus Magazine, Issue 19,September, 2010)

• Department of Chemistry, US Airforce Academy, USA

  Barry W. Hicks

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