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DNA and RNA Profiling in Human Blood

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Peter Bugert

    1. Institute of Transfusion Medicine and Immunology, Heidelberg University Medical Faculty of Mannheim, Germany

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  • Details numerous techniques to perform blood group genotyping, covering prenatal blood grouping from maternal plasma to multiplex HLA genotyping
  • Presents not only established but also cutting-edge developed protocols for complex and high-throughput DNA and RNA profiling
  • Covers low- to high-throughput methods for SNP typing such as pyrosequencing or microbeads
  • Includes protocols for RNA profiling by modern PCR and bead chip approaches
  • Provides an overview of up-to-date DNA and RNA profiling technologies with plenty of methodological details to help you to establish protocols in your own lab

Part of the book series: Methods in Molecular Biology (MIMB, volume 496)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. DNA Profiling for Blood Cell Antigens

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. PCR–ELISA for High-Throughput Blood Group Genotyping

      • Maryse St-Louis
      Pages 3-13

    3. Single Base Extension in Multiplex Blood Group Genotyping

      • Gregory A. Denomme
      Pages 15-24

    4. Real-Time PCR Assays for High-Throughput Blood Group Genotyping

      • Fernando Araujo
      Pages 25-37

    5. Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing

      • Simon E. McBride
      Pages 39-49

    6. Multiplex ABO Genotyping by Minisequencing

      • Gianmarco Ferri, Susi Pelotti
      Pages 51-58

    7. Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays

      • Susan Bortolin
      Pages 59-72

    8. Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance

      • Parviz Ahmad-Nejad
      Pages 73-87

    9. Multiplex HLA-Typing by Pyrosequencing

      • Ying Lu, Julian Boehm, Lynn Nichol, Massimo Trucco, Steven Ringquist
      Pages 89-114

    10. High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach

      • Clarissa Consolandi
      Pages 115-127

    11. Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads

      • Charles H. Lin, Joanne M. Yeakley, Timothy K. McDaniel, Richard Shen
      Pages 129-142

    12. The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping

      • Kirstin Finning, Pete Martin, Geoff Daniels
      Pages 143-157

  3. RNA Profiling in Blood Cells

    1. Front Matter

      Pages 159-159
      Download chapter PDF

    2. Nanoliter High-Throughput PCR for DNA and RNA Profiling

      • Colin J. H. Brenan, Douglas Roberts, James Hurley
      Pages 161-174

    3. RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis

      • Svenja Debey-Pascher, Daniela Eggle, Joachim L. Schultze
      Pages 175-210

    4. RNA Profiling in Peripheral Blood Cells by Fluorescent Differential Display PCR

      • Martin Steinau, Mangalathu S. Rajeevan
      Pages 211-222

    5. cDNA Amplification by SMART-PCR and Suppression Subtractive Hybridization (SSH)-PCR

      • Andrew Hillmann, Eimear Dunne, Dermot Kenny
      Pages 223-243

    6. Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGE)

      • Dmitri V. Gnatenko, John J. Dunn, John Schwedes, Wadie F. Bahou
      Pages 245-272

    7. Genome-Wide Platelet RNA Profiling in Clinical Samples

      • Angelika Schedel, Nina Rolf
      Pages 273-283
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Keywords

About this book

Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes. In DNA and RNA Profiling in Human Blood: Methods and Protocols, leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful Methods in Molecular Biology™ series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA and RNA Profiling in Human Blood: Methods and Protocols is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and have shown great promise in the further identifying of disease markers in blood.

Editors and Affiliations

  • Institute of Transfusion Medicine and Immunology, Heidelberg University Medical Faculty of Mannheim, Germany

    Peter Bugert

Bibliographic Information

  • Book Title: DNA and RNA Profiling in Human Blood

  • Book Subtitle: Methods and Protocols

  • Editors: Peter Bugert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-553-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Softcover ISBN: 978-1-62703-913-0Published: 08 April 2015

  • eBook ISBN: 978-1-59745-553-4Published: 08 October 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 341

  • Number of Illustrations: 62 b/w illustrations, 1 illustrations in colour

  • Topics: Human Genetics

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