Overview
- An easily accessible reference volume for procedures on how to identify, characterize and measure Toll-like receptor expression and downstream signalling
- Includes step-by-step methods on the most up-to-date techniques such as FRET microscopy, GFP fragmentation, microarray design and analysis and 2D-DIGE comparative proteomics
- Illustrates how genetic analysis can uncover Toll-like receptor function using methods such as siRNA, ENU mutagenesis and polymorphism genotyping
- Details the most current disease models used to investigate Toll-like receptor contribution
Part of the book series: Methods in Molecular Biology (MIMB, volume 517)
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Table of contents(25 protocols)
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Methods to Detect and Analyze Toll-Like Receptors
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Methods to Analyze Signal Transduction Downstream of Toll-Like Receptor Stimulation
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Genetic Techniques in Toll-Like Receptor Analysis
About this book
In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories. In Toll-Like Receptors: Methods and Protocols, experts in the field contribute techniques currently used to study TLRs, their downstream signalling pathways, and their role in the pathogenesis of disease, with sections examining TLR expression and the application of genetic techniques and microarray analysis to TLR research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections highlighting tips on troubleshooting and avoiding known pitfalls.
Versatile and cutting-edge, Toll-Like Receptors: Methods and Protocols provides an ideal manual for a wide range of biologists and medical researchers studying these vital components of the immune system.
Reviews
Editors and Affiliations
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School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland
Claire E. McCoy, Luke A. J. O’Neill
Bibliographic Information
Book Title: Toll-Like Receptors
Book Subtitle: Methods and Protocols
Editors: Claire E. McCoy, Luke A. J. O’Neill
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-541-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-934115-72-5
Softcover ISBN: 978-1-61737-942-0
eBook ISBN: 978-1-59745-541-1
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XXIV, 376
Number of Illustrations: 66 b/w illustrations, 5 illustrations in colour
Topics: Immunology, Cytokines and Growth Factors