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Toll-Like Receptors

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Claire E. McCoy

    1. School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland

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    You can also search for this editor in PubMed   Google Scholar

  2. Luke A. J. O’Neill

    1. School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • An easily accessible reference volume for procedures on how to identify, characterize and measure Toll-like receptor expression and downstream signalling
  • Includes step-by-step methods on the most up-to-date techniques such as FRET microscopy, GFP fragmentation, microarray design and analysis and 2D-DIGE comparative proteomics
  • Illustrates how genetic analysis can uncover Toll-like receptor function using methods such as siRNA, ENU mutagenesis and polymorphism genotyping
  • Details the most current disease models used to investigate Toll-like receptor contribution

Part of the book series: Methods in Molecular Biology (MIMB, volume 517)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Methods to Detect and Analyze Toll-Like Receptors

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Expression Analysis of the Toll-Like Receptors in Human Peripheral Blood Mononuclear Cells

      • Jakub Siednienko, Sinead M. Miggin
      Pages 3-14

    3. Ligands, Cell-Based Models, and Readouts Required for Toll-Like Receptor Action

      • Jérôme Dellacasagrande
      Pages 15-32

    4. Toll-Like Receptor Interactions Imaged by FRET Microscopy and GFP Fragment Reconstitution

      • Gabor Horvath, Scott Young, Eicke Latz
      Pages 33-54

    5. Predicting Toll-Like Receptor Structures and Characterizing Ligand Binding

      • Joshua N. Leonard, Jessica K. Bell, David M. Segal
      Pages 55-67

    6. Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures

      • Tom P. Monie, Nicholas J. Gay, Monique Gangloff
      Pages 69-79

    7. Expression, Purification, and Crystallization of Toll/Interleukin-1 Receptor (TIR) Domains

      • Xiao Tao, Liang Tong
      Pages 81-88

  3. Methods to Analyze Signal Transduction Downstream of Toll-Like Receptor Stimulation

    1. Front Matter

      Pages 89-89
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    2. Proteomic Analysis of Protein Complexes in Toll-Like Receptor Biology

      • Kiva Brennan, Caroline A. Jefferies
      Pages 91-104

    3. 2D-DIGE: Comparative Proteomics of Cellular Signalling Pathways

      • Nadia Ben Larbi, Caroline Jefferies
      Pages 105-132

    4. MAPPIT (Mammalian Protein–Protein Interaction Trap) Analysis of Early Steps in Toll-Like Receptor Signalling

      • Peter Ulrichts, Irma Lemmens, Delphine Lavens, Rudi Beyaert, Jan Tavernier
      Pages 133-144

    5. Analysis of the Functional Role of Toll-Like Receptor-4 Tyrosine Phosphorylation

      • Andrei E. Medvedev, Wenji Piao
      Pages 145-167

    6. Analysis of Ubiquitin Degradation and Phosphorylation of Proteins

      • Pearl Gray
      Pages 169-190

    7. The Generation of Highly Purified Primary Human Neutrophils and Assessment of Apoptosis in Response to Toll-Like Receptor Ligands

      • Lisa C. Parker, Lynne R. Prince, David J. Buttle, Ian Sabroe
      Pages 191-204

    8. Cellular Expression of A20 and ABIN-3 in Response to Toll-Like Receptor-4 Stimulation

      • Kelly Verhelst, Lynn Verstrepen, Beatrice Coornaert, Isabelle Carpentier, Rudi Beyaert
      Pages 205-215

    9. Characterisation of Viral Proteins that Inhibit Toll-Like Receptor Signal Transduction

      • Julianne Stack, Andrew G. Bowie
      Pages 217-235

  4. Genetic Techniques in Toll-Like Receptor Analysis

    1. Front Matter

      Pages 237-237
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    2. Genetic Dissection of Toll-Like Receptor Signaling Using ENU Mutagenesis

      • Kasper Hoebe
      Pages 239-251

    3. Microarray Experiments to Uncover Toll-Like Receptor Function

      • Harry Björkbacka
      Pages 253-275
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Keywords

About this book

In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories. In Toll-Like Receptors: Methods and Protocols, experts in the field contribute techniques currently used to study TLRs, their downstream signalling pathways, and their role in the pathogenesis of disease, with sections examining TLR expression and the application of genetic techniques and microarray analysis to TLR research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections highlighting tips on troubleshooting and avoiding known pitfalls.

Versatile and cutting-edge, Toll-Like Receptors: Methods and Protocols provides an ideal manual for a wide range of biologists and medical researchers studying these vital components of the immune system.

Reviews

From the reviews: “This is a condensed and concise account of the various techniques used to study toll-like receptors, their downstream signaling pathways, and their role in the pathogenesis of disease. … In addition to biologists and medical researchers, students, residents, and practitioners with an interest in the fields of bacteriology and immunology … find this book interesting. … It is adequately illustrated with black-and-white figures and color plates which readers will find useful.” (Omer Iqbal, Doody’s Review Service, July, 2009) “The book ‘Toll-Like Receptors: Methods and Protocols’ with 65 contributors, focuses on recent developments of TLRs investigations performed by many institutions including Universities, Technical Universities and Medical Schools in Europe and USA. … Experimental tips and troubleshooting procedures are also included, which is invaluable for beginners in the field. … an excellent manual for all investigators as well as teachers and advanced students of biological and medical sciences interested in modern immunology. … fills an informational gap in very vital field of knowledge.” (Elżbieta Wałajtys-Rode, Acta Biochimica Polonica, Vol. 56, December, 2009) “This book is another protocols book in the successful Humana Press Methods in Molecular Biology series. It is specifically aimed at biologists and medical researchers active in the Toll-like receptor (TLR) research field or those who will develop an interest in TLR research in the future. … this book has been well-targeted and specifically tailored to the researcher, but in a language that makes it accessible and easy to understand for final year undergraduate and post-graduate students.” (Andrew Foey, Immunology News, November, 2009)

Editors and Affiliations

  • School of Biochemistry and Immunology, Trinity College Dublin, Dublin 2, Ireland

    Claire E. McCoy, Luke A. J. O’Neill

Bibliographic Information

  • Book Title: Toll-Like Receptors

  • Book Subtitle: Methods and Protocols

  • Editors: Claire E. McCoy, Luke A. J. O’Neill

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-541-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-934115-72-5Published: 01 April 2009

  • Softcover ISBN: 978-1-61737-942-0Published: 26 January 2011

  • eBook ISBN: 978-1-59745-541-1Published: 20 April 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXIV, 376

  • Number of Illustrations: 66 b/w illustrations, 5 illustrations in colour

  • Topics: Immunology, Cytokines and Growth Factors

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