Skip to main content
SpringerLink
Log in

Yeast Functional Genomics and Proteomics

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Igor Stagljar
    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Covers a wide range of state-of-the-art yeast-based functional genomics and proteomics technologies
  • Provides an easily accessible reference volume for most important yeast-based functional genomics and proteomics technologies written by leading researchers in the field
  • Comprehensive guide to running and analyzing yeast-based assays in functional genomics and proteomics
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 548)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (15 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Comparative Genome Hybridization on Tiling Microarrays to Detect Aneuploidies in Yeast

    • Barry Dion, Grant W. Brown
    Pages 1-18

  3. Identification of Transcription Factor Targets by Phenotypic Activation and Microarray Expression Profiling in Yeast

    • Gordon Chua
    Pages 19-35

  4. SGAM: An Array-Based Approach for High-Resolution Genetic Mapping in Saccharomyces cerevisiae

    • Michael Costanzo, Charles Boone
    Pages 37-53

  5. Reporter-Based Synthetic Genetic Array Analysis: A Functional Genomics Approach for Investigating the Cell Cycle in Saccharomyces cerevisiae

    • Holly E. Sassi, Nazareth Bastajian, Pinay Kainth, Brenda J. Andrews
    Pages 55-73

  6. The Fidgety Yeast: Focus on High-Resolution Live Yeast Cell Microscopy

    • Heimo Wolinski, Klaus Natter, Sepp D. Kohlwein
    Pages 75-99

  7. A Genomic Approach to Yeast Chronological Aging

    • Christopher R. Burtner, Christopher J. Murakami, Matt Kaeberlein
    Pages 101-114

  8. Chemogenomic Approaches to Elucidation of Gene Function and Genetic Pathways

    • Sarah E. Pierce, Ronald W. Davis, Corey Nislow, Guri Giaever
    Pages 115-143

  9. Identification of Inhibitors of Chromatin Modifying Enzymes Using the Yeast Phenotypic Screens

    • Benjamin Newcomb, Antonio Bedalov
    Pages 145-160

  10. Exploiting Yeast Genetics to Inform Therapeutic Strategies for Huntington’s Disease

    • Flaviano Giorgini, Paul J. Muchowski
    Pages 161-174

  11. Global Proteomic Analysis of Saccharomyces cerevisiae Identifies Molecular Pathways of Histone Modifications

    • Jessica Jackson, Ali Shilatifard
    Pages 175-186

  12. Systematic Characterization of the Protein Interaction Network and Protein Complexes in Saccharomyces cerevisiae Using Tandem Affinity Purification and Mass Spectrometry

    • Mohan Babu, Nevan J. Krogan, Donald E. Awrey, Andrew Emili, Jack F. Greenblatt
    Pages 187-207

  13. Protein Microarrays

    • Joseph Fasolo, Michael Snyder
    Pages 209-222

  14. Analysis of Protein–Protein Interactions Using Array-Based Yeast Two-Hybrid Screens

    • Seesandra V. Rajagopala, Peter Uetz
    Pages 223-245

  15. Analysis of Membrane Protein Complexes Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System

    • Saranya Kittanakom*, Matthew Chuk*, Victoria Wong, Jamie Snyder, Dawn Edmonds, Apostolos Lydakis et al.
    Pages 247-271

  16. Computational Analysis of the Yeast Proteome: Understanding and Exploiting Functional Specificity in Genomic Data

    • Curtis Huttenhower, Chad L. Myers, Matthew A. Hibbs, Olga G. Troyanskaya
    Pages 273-293

  17. Back Matter

    Pages 295-300
    Download chapter PDF
Back to top

Keywords

About this book

Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. As part of the highly successful Methods in Molecular Biology™ series, the chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easy to use, Yeast Functional Genomics and Proteomics: Methods and Protocols is an ideal reference for both yeast researchers and those who wish to use yeast as a model system for the further study of functional genomics and proteomics.

Reviews

From the reviews: “This volume takes a snapshot of the questions which can be asked with a genome … . A major value of this book may be in helping to overcome the fear factor. Most of the approaches are feasible in a moderately resourced laboratory, and as the tools for S. cerevisiae spread to other organisms, so will the potential usefulness of this collection.” (John Armstrong, Microbiology Today, January, 2010)

Bibliographic Information

  • Book Title: Yeast Functional Genomics and Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Igor Stagljar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-540-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-934115-71-8Published: 17 June 2009

  • Softcover ISBN: 978-1-62703-959-8Published: 26 November 2014

  • eBook ISBN: 978-1-59745-540-4Published: 12 June 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 304

  • Topics: Mycology, Microbial Genetics and Genomics, Proteomics

Publish with us

Policies and ethics

Back to top

Search

Navigation