Overview
- Editors:
-
-
Mohan C. Vemuri
-
Stem Cells, Reprogenetics, West Orange
Invitrogen Corporation, Grand Island
- Brings recent developments to the forefront in a timely manner
- Presents scientific advances in stem cell methods for a wider use by novice and expert scientists
Access this book
Other ways to access
Table of contents (24 protocols)
-
-
- Mohan C. Vemuri, Tim Schimmel, Pere Colls, Santiago Munne, Jacques Cohen
Pages 1-10
-
-
- A. Henry Sathananthan, Stefania A. Nottola
Pages 21-41
-
- Carol B. Ware, Szczepan W. Baran
Pages 43-49
-
- Raj R. Rao, Alison Venable Johnson, Steven L. Stice
Pages 51-61
-
- Micha Drukker, Christina Muscat, Irving L. Weissman
Pages 63-81
-
-
- Uma Lakshmipathy, Brad Love, Christopher Adams, Bhaskar Thyagarajan, Jonathan D. Chesnut
Pages 97-114
-
- Uma Lakshmipathy, Shannon Buckley, Catherine Verfaillie
Pages 115-126
-
- Gang-Ming Zou, Meredith A. Thompson, Mervin C. Yoder
Pages 127-136
-
- Christopher J. Porter, Gareth A. Palidwor, Reatha Sandie, Paul M. Krzyzanowski, Enrique M. Muro, Carolina Perez-Iratxeta et al.
Pages 137-148
-
- Nishanth P. Reddy, Mohan C. Vemuri, Reddanna Pallu
Pages 149-163
-
- Chao-Ling Yao, Shiaw-Min Hwang
Pages 165-175
-
- Carla Pereira, Emer Clarke, Jackie Damen
Pages 177-208
-
-
- Panagiota A. Sotiropoulou, Michael Papamichail
Pages 225-243
-
- Panagiota A. Sotiropoulou, Sonia A. Perez, Michael Papamichail
Pages 245-263
-
- Pablo Bosch, Steven L. Stice
Pages 265-274
-
- Maxim A. Vodyanik, Igor I. Slukvin
Pages 275-293
About this book
Stem cells and regenerative medicine is a fast emerging field with rapid strides of progress and focus on human health. Successful utilization of stem cells in regenerative medicine depends on two important features. One, stem cells can grow and divide indefinitely, and two, stem cells can differentiate into specialized cell types that make up tissues such as pancreas, heart, liver, blood, and others. Utilizing the property of differentiation, stem cells can be engineered to produce cell types to replace wornout cells and to regenerate damaged tissue. The scope for improving healthcare using stem cell therapies is thrilling, but considerable technical challenges and methodological constraints need to be addressed. There is a great need for isolation and propagation of “transplant-ready” or “clinical-grade” stem cells and screening methods, well before they are used in therapies. Recent scientific advances have contributed enormously to the development of new methods pertaining to both embryonic and adult stem cell isolation, characterization, expansion in undifferentiated state, customized differentiation, efficient stem cell transduction for gene therapy, quick monitoring of rejection, through polymerase chain reaction approaches following transplantation, and more. The scientific progress that makes stem cell biology so exciting also threatens to suffocate researchers with an avalanche of information. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, the book Stem Cell Assays aims to present scientific advances in stem cell methods for a wider use by novice and expert scientists, through the Methods in Molecular Biology series.
Reviews
From the reviews:
"The present volume ‘Stem Cell Assays’ … include very detailed experimental sections, which are essentially raw lab protocols. … All protocols are explained in full detail … and should be very useful for researchers novel to this field. In addition, for more experienced scientists it may be very useful to compare their own protocols with those provided in this volume … . this book will be of great value for experimental scientists that wish to introduce novel technology into their labs." (Gerald de Haan, Molecular Biotechnology, May, 2008)
Editors and Affiliations
-
Stem Cells, Reprogenetics, West Orange
Mohan C. Vemuri
-
Invitrogen Corporation, Grand Island
Mohan C. Vemuri
