Overview
- Cutting-edge methods for genome-scale experiments and small-scale individual PCR amplifications
- Highlights basic principles and software for PCR primer design
- An exceptional reference for all those involved in studying the genome
Part of the book series: Methods in Molecular Biology (MIMB, volume 402)
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Table of contents (21 protocols)
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Front Matter
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Basic Principles and Software for PCR Primer Design
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Front Matter
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Genome-Scale PCR Primer Design
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Front Matter
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Repeat Masking for PCR Primer Design
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Front Matter
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Repeat Masking for PCR Primer Design
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Multiplex PCR Primer Design
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Front Matter
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Keywords
About this book
In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome.
In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists – geneticists, molecular biologists, molecular geneticists, and more – who routinely use PCR in their research.
Editors and Affiliations
Bibliographic Information
Book Title: PCR Primer Design
Editors: Anton Yuryev
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-528-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-725-9Published: 02 August 2007
Softcover ISBN: 978-1-61737-743-3Published: 19 November 2010
eBook ISBN: 978-1-59745-528-2Published: 03 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 432
Number of Illustrations: 128 b/w illustrations
Topics: Cell Biology, Human Genetics