Meiosis

1. Front Matter

   Pages i-xi

   PDF

2. Genetic Methods for Studying Meiotic Recombination and Chromosome Dynamics

   1. Front Matter

      Pages 1-1

      PDF

   2. Interaction of Genetic and Environmental Factors in Saccharomyces cerevisiae Meiosis: The Devil is in the Details

      • Victoria E. Cotton, Eva R. Hoffmann, Mohammed F.F. Abdullah, Rhona H. Borts

      Pages 3-20

   3. Optimizing Sporulation Conditions for Different Saccharomyces cerevisiae Strain Backgrounds

      • Susan L. Elrod, Sabrina M. Chen, Katja Schwartz, Elizabeth O. Shuster

      Pages 21-26

   4. Modulating and Targeting Meiotic Double-Strand Breaks in Saccharomyces cerevisiae

      • Alain Nicolas

      Pages 27-33

   5. Methods for Analysis of Crossover Interference in Saccharomyces cerevisiae

      • Franklin W. Stahl, Elizabeth A. Housworth

      Pages 35-53

   6. Measurement of Spatial Proximity and Accessibility of Chromosomal Loci in Saccharomyces cerevisiae Using Cre /loxP Site-Specific Recombination

      • Doris Lui, Sean M. Burgess

      Pages 55-63

   7. Genetic Analysis of Meiotic Recombination in Schizosaccharomyces pombe

      • Gerald R. Smith

      Pages 65-76

   8. Analysis of Meiotic Recombination in Caenorhabditis elegans

      • Kenneth J. Hillers, Anne M. Villeneuve

      Pages 77-97

   9. Visual Markers for Detecting Gene Conversion Directly in the Gametes of Arabidopsis thaliana

      • Luke E. Berchowitz, Gregory P. Copenhaver

      Pages 99-114

3. Molecular Analysis of Recombination and Protein-DNA Interactions During Meiosis

   1. Front Matter

      Pages 115-115

      PDF

   2. Gel Electrophoresis Assays for Analyzing DNA Double-Strand Breaks in Saccharomyces cerevisiae at Various Spatial Resolutions

      • Hajime Murakami, Valérie Borde, Alain Nicolas, Scott Keeney

      Pages 117-142

   3. Genome-Wide Mapping of Meiotic DNA Double-Strand Breaks in Saccharomyces cerevisiae

      • Cyril Buhler, Robert Shroff, Michael Lichten

      Pages 143-164

   4. Detection of Meiotic DNA Breaks in Mouse Testicular Germ Cells

      • Jian Qin, Jaichandar Subramanian, Norman Arnheim

      Pages 165-181

   5. End-Labeling and Analysis of Spo11-Oligonucleotide Complexes in Saccharomyces cerevisiae

      • Matthew J. Neale, Scott Keeney

      Pages 183-195

   6. Detection of SPO11-Oligonucleotide Complexes from Mouse Testes

      • Jing Pan, Scott Keeney

      Pages 197-207

   7. Stabilization and Electrophoretic Analysis of Meiotic Recombination Intermediates in Saccharomyces cerevisiae

      • Steve D. Oh, Lea Jessop, Jessica P. Lao, Thorsten Allers, Michael Lichten, Neil Hunter

      Pages 209-234

   8. Using Schizosaccharomyces pombe Meiosis to Analyze DNA Recombination Intermediates

      • Randy W. Hyppa, Gerald R. Smith

      Pages 235-252

   9. Analysis of Chromatin Structure at Meiotic DSB Sites in Yeasts

      • Kouji Hirota, Tomoyuki Fukuda, Takatomi Yamada, Kunihiro Ohta

      Pages 253-266

   10. Analysis of Protein–DNA Interactions During Meiosis by Quantitative Chromatin Immunoprecipitation (qChIP)

       • Marco Antonio Mendoza, Silvia Panizza, Franz Klein

       Pages 267-283

Each generation in a sexually reproducing organism such as a fly or a mouse passes through the bottleneck of meiosis, which is the specialized cell division that gives rise to haploid reproductive cells (sperm, eggs, spores, etc. ). The principal function of meiosis is to reduce the genome complement by half, which is accomplished through sequential execution of one round of DNA replication followed by two rounds of chromosome segregation. Within the extended prophase between DNA replication and the first meiotic division in most organisms, homologous maternal and paternal chromosomes pair with one another and undergo homologous recombination, which establishes physical connections that link the homologous chromosomes until the time they are separated at anaphase I. Recombination also serves to increase genetic diversity from one generation to the next by breaking up linkage groups. The unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been an explosion of new information about how meiotic chromosomes pair, recombine, and are segregated. Progress has been driven by advances in three main areas: (1) genetic identification of meiosis-defective mutants and cloning of the genes involved; (2) development of direct physical assays for DNA intermediates and products of recombination; and (3) increasingly sophisticated cy- logical methods that describe chromosome behaviors and the spatial and temporal patterns by which specific proteins associate with meiotic chromosomes.

• Arabidopsis thaliana
• Budding and fission yeasts
• Chromosome dynamics
• DNA
• Meiotic recombination
• Protein-DNA interactions
• genes
• molecular biology
• mouse
• proteins
• saccharomyces cerevisiae

• Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, U.S.A.

  Scott Keeney

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