Overview
- Contains a comprehensive set of methods for developing transgenic maize varieties, including construct design, transformation, analysis, and breeding
- Includes methods in tissue culture, molecular biology, biochemistry, genetics, and agronomy
- Presents data from case studies of research with transgenic maize
- Appendices provide examples of regulatory paperwork required by the US government for experimental production of transgenic maize
- Displays full-color plates to illustrate the utility of fluorescent marker proteins
- Allows readers to compare transformation methods used by public and private sector laboratories
Part of the book series: Methods in Molecular Biology (MIMB, volume 526)
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Table of contents (14 protocols)
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Front Matter
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Introduction
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Front Matter
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Analysis of Transgenic Plants
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Front Matter
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Breeding with Transgenes
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Front Matter
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Keywords
About this book
As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.
Reviews
From the reviews:
“This book is number 526 in the popular ‘Methods in Molecular Biology’ series, which is renowned for its targeted protocol chapters, each with photographs, recipes and a set of notes for additional practical guidance and to avoid common pitfalls. … There is a lot to like about this collection of practical chapters, which represent an excellent investment for the applied maize researcher, who could be a student doing an undergraduate project through to a professional scientist in a dedicated transformation laboratory.” (Huw D. Jones, Annals of Botany, Vol. 109 (7), June, 2012)Editors and Affiliations
Bibliographic Information
Book Title: Transgenic Maize
Book Subtitle: Methods and Protocols
Editors: M. Paul Scott
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-494-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-934115-49-7Published: 05 March 2009
Softcover ISBN: 978-1-61737-935-2Published: 19 November 2010
eBook ISBN: 978-1-59745-494-0Published: 20 April 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: IX, 188
Topics: Agriculture, Plant Sciences, Plant Genetics and Genomics, Transgenics