Overview
- Editors:
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Sean Cutler
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Dept. Botany, University of Toronto, Toronto, Canada
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Dario Bonetta
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Dept. Botany, University of Toronto, Toronto, Canada
- Cutting edge approaches for studying plant hormones and their receptors that have been perfected over the last 5 year
- Provides the basics of the LC-MS and GC-MS methods that are critical to modern hormone research
- Includes advanced protocols for mutant identification and assaying hormone responses in heterologous systems such as yeast
- Offers a concise overview of current methods and reagents needed to dissect plant signalling pathways using chemical genetic methods
- Features robust protocols for the use of high-throughput sequencing technologies for microRNA analysis
- Includes supplementary material: sn.pub/extras
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Table of contents (12 protocols)
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Front Matter
Pages i-viii
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- Julian Northey, Peter McCourt
Pages 1-10
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- Danielle Marcos, Thomas Berleth
Pages 11-20
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- George W. Bassel, Nicholas J. Provart
Pages 21-37
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- Stacey J. Owen, Suzanne R. Abrams
Pages 39-51
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- Masanori Okamoto, Atsushi Hanada, Yuji Kamiya, Shinjiro Yamaguchi, Eiji Nambara
Pages 53-60
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- Ana Caño-Delgado, Zhi-Yong Wang
Pages 81-88
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- Fawzi A. Razem, Robert D. Hill
Pages 89-99
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- Masayuki Higuchi, Tatsuo Kakimoto, Takeshi Mizuno
Pages 101-109
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- Georgy A. Romanov, Sergey N. Lomin
Pages 111-120
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- Padmanabhan Chellappan, Hailing Jin
Pages 121-132
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- Lorena Norambuena, Glenn R. Hicks, Natasha V. Raikhel
Pages 133-143
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Back Matter
Pages 145-146
About this book
The last 10 years have witnessed an explosion in our understanding of plant h- mones. The often vague models of hormone action developedover decadeshave been replaced in short order by detailed molecular models that include receptors and in many cases downstream signal transduction components. Given the rapid progress in understanding the mechanism of action of plant growth regulators, a technical review of hormone methodology is timely. Our book focuses on genetic, biochemical, ana- tical and chemical biological approaches for understanding and dissecting plant h- mone action. The greatest strides in plant hormone biology have come, by and large, from the use of genetic methods to identify receptors and we dedicate a chapter to general genetic methods of analysis using the model system Arabidopsis thaliana. A cluster of chapters focuses on biochemical methods for documenting interactions betweenhormonesand their receptors. Theimportance of these assays is tremendous; receptor–ligand interactions in animal model systems have been the cornerstones of pharmacological and medicinal chemical assays that have enabled identification of selective and non-selective agonists and antagonists that can be used to further probe and dissect questions of receptor function. This is likely to be a major new frontier in plant hormone research.
Reviews
From the reviews of the second edition:
“This book introduces several approaches essential for plant hormone biology at the forefront of research. … This book has been published at an apt time for plant hormone biologists, especially for PhD students and advanced researchers in this field, to move the field to a higher stage and to lead them towards wider and more advanced technical approaches to hormone analyses, to encourage them to accumulate more specific genomic information and to apply more sophisticated analytical tools.” (Tomokazu Koshiba, Annals of Botany, Vol. 105 (4), April, 2010)