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Neutrophil Methods and Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Mark T. Quinn

    1. Deparment of Veterinary Molecular Biology, Montana State University, Bozeman

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  2. Frank R. DeLeo

    1. Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton

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    You can also search for this editor in PubMed   Google Scholar

  3. Gary M. Bokoch

    1. Departments of Immunology and Cell Biology, The Scripps Institute, La Jolla

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  • Provides neutrophil protocols that are broadly applicable to leukocyte biology
  • Includes protocols for isolation of neutrophils from humans and animal species
  • Presents an interesting and informative overview of the field of neutrophil biology
  • Facilitates a better understanding of the role of neutrophils in host defense

Part of the book series: Methods in Molecular Biology (MIMB, volume 412)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xx
    Download chapter PDF

  2. Neutrophils: An Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Role of Neutrophils in the Immune System

      • Harry L. Malech
      Pages 3-11

  3. Neutrophil Isolation and Subcellular Fractionation

    1. Front Matter

      Pages 13-13
      Download chapter PDF

    2. Isolation of Human Neutrophils From Venous Blood

      • William M. Nauseef
      Pages 15-20

    3. Neutrophil Isolation From Nonhuman Species

      • Daniel W. Siemsen, Igor A. Schepetkin, Liliya N. Kirpotina, Benfang Lei, Mark T. Quinn
      Pages 21-34

    4. Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers

      • Lene Udby, Niels Borregaard
      Pages 35-56

  4. Biochemistry, Biology, and Signal Transduction of Neutrophils

    1. Front Matter

      Pages 57-57
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    2. Rac and Rap GTPase Activation Assays

      • Ulla G. Knaus, Alison Bamberg, Gary M. Bokoch
      Pages 59-67

    3. Measurement of Phospholipid Metabolism in Intact Neutrophils

      • Susan Sergeant, Linda C. McPhail
      Pages 69-83

    4. Analysis of Protein Phosphorylation in Human Neutrophils

      • Jamel El-Benna, Pham My-Chan Dang
      Pages 85-96

    5. Mitogen-Activated Protein Kinase Assays

      • Martine Torres
      Pages 97-113

    6. Transduction of Proteins into Intact Neutrophils

      • Tieming Zhao, Gary M. Bokoch
      Pages 115-123

    7. Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils

      • Esther J. Hillson, Sharon Dewitt, Maurice B. Hallett
      Pages 125-137

    8. Analysis of Electrophysiological Properties and Responses of Neutrophils

      • Deri Morgan, Thomas E. DeCoursey
      Pages 139-175

    9. Analysis of Neutrophil Apoptosis

      • Emma L. Taylor, Adriano G. Rossi, Ian Dransfield, Simon P. Hart
      Pages 177-200

  5. Neutrophil Adhesion and Chemotaxis

    1. Front Matter

      Pages 201-201
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    2. Optical and Fluorescence Detection of Neutrophil Integrin Activation

      • Ulrich Y. Schaff, Melissa R. Sarantos, Harold Ting, Scott I. Simon
      Pages 203-210

    3. Analysis of Neutrophil Polarization and Chemotaxis

      • Mary A. Lokuta, Paul A. Nuzzi, Anna Huttenlocher
      Pages 211-229

    4. Quantifying and Localizing Actin-Free Barbed Ends in Neutrophils

      • Michael Glogauer
      Pages 231-237
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Keywords

About this book

Neutrophil Methods and Protocols provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Parts 1 & 2 provide an overview of neutrophils the methods most commonly used for isolating them from humans and other animal species. Part 3 details methods for investigating adhesion, chemotaxis, phagocytosis and bacterial activity. Part 4 presents protocols measuring gene expression in neutrophils, and Part 5 concludes with assays for diagnosing the most common neutrophil disorders. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.

Reviews

From the reviews:

"...a carefully compiled collection of protocols that allow for study of many aspects of the neutrophil." -Doody's Book Review, Weighted Numerical Score:85-3 Stars

"This excellent book is one of the latest in the ‘Methods in Molecular Biology’ series … . it comes beautifully presented and bound in a hardback cover and would grace any bookshelf. I would urge all self-respecting laboratories working with neutrophils – whether in research or diagnostics – to purchase one immediately. … I was impressed by this book’s comprehensive provision of protocols, addressing all aspects of neutrophil function and physiology, and including an excellent section on diagnosis of both inherited and non-inherited neutrophil disorders." (Lynn Morgan, Immunology News, May, 2008)

Editors and Affiliations

  • Deparment of Veterinary Molecular Biology, Montana State University, Bozeman

    Mark T. Quinn

  • Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton

    Frank R. DeLeo

  • Departments of Immunology and Cell Biology, The Scripps Institute, La Jolla

    Gary M. Bokoch

Bibliographic Information

  • Book Title: Neutrophil Methods and Protocols

  • Editors: Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-467-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-788-4Published: 02 August 2007

  • Softcover ISBN: 978-1-61737-779-2Published: 10 December 2010

  • eBook ISBN: 978-1-59745-467-4Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 537

  • Number of Illustrations: 104 b/w illustrations, 8 illustrations in colour

  • Topics: Immunology, Cell Biology, Human Genetics

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