Editors:
- Includes useful tips for baculovirus research and detailed procedures for quantifying cell metabolic behavior
- Lists all available insect cell lines and provides methods for isolating new cell lines
- Numerous methods and protocols to inspire new techniques and applications for baculovirus and insect cell culture
Part of the book series: Methods in Molecular Biology (MIMB, volume 388)
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Table of contents (25 protocols)
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Front Matter
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Introduction
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Front Matter
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Baculovirus Molecular Biology/Development of Recombinant Baculoviruses
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Front Matter
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Protein Production with Recombinant Baculoviruses
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Front Matter
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Recombinant Protein Production with Transformed Insect Cells
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Front Matter
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About this book
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology™ series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.
Editors and Affiliations
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Department of Chemical and Biochemical Engineering, The University of Iowa, Iowa City
David W. Murhammer
Bibliographic Information
Book Title: Baculovirus and Insect Cell Expression Protocols
Editors: David W. Murhammer
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-457-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-537-8Published: 06 July 2007
Softcover ISBN: 978-1-61737-629-0Published: 19 November 2010
eBook ISBN: 978-1-59745-457-5Published: 03 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 470
Topics: Microbiology, Biochemistry, general, Plant Biochemistry, Cell Biology, Plant Sciences