Overview
- All protocols are tested and readily and reproducible
- Updates and expands upon the first volume
- Presents the basics of Pichia pastoris and describes available vectors and host strains for expression
Part of the book series: Methods in Molecular Biology (MIMB, volume 389)
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Table of contents (18 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Pichia Protocols focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI).
This volume fully updates and expands upon the first edition of Pichia Protocols, and focuses primarily on information that has come to light since its original publication. Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system.
Reviews
From the reviews of the second edition:
"P. pastoris has many superior traits. … can grow to very high cell densities prior to switching on an inducible promoter (the AOX1 gene) for maximal recombinant protein production. The diversity of such proteins produced by expression strains of P. pastoris is quite amazing and this book describes detailed experimental protocols for a few of them (e.g. hookworm protein, botulinum toxin). … This is a stand-alone book … . a book that will prove valuable to yeast molecular geneticists and biotechnologists." (Graeme Walker, Microbiology Today, May, 2008)
Editors and Affiliations
Bibliographic Information
Book Title: Pichia Protocols
Editors: James M. Cregg
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-456-8
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-429-6Published: 08 August 2007
Softcover ISBN: 978-1-61737-563-7Published: 19 November 2010
eBook ISBN: 978-1-59745-456-8Published: 03 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 268
Topics: Biotechnology, Cell Biology, Microbial Genetics and Genomics, Biological Microscopy