Overview
- Includes state-of-the-art procedures for isolating and working with nuclei from a wide range of cell types, and
- procedures for studying their components including pathology-related structures
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 463)
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Table of contents (19 protocols)
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Front Matter
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The Intranuclear Environment
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Front Matter
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Isolation of Nuclei
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Front Matter
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The Nucleolus
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Front Matter
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Intranuclear Structures
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Front Matter
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Interphase Chromosomes
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Front Matter
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Keywords
About this book
Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume I: Nuclei and Subnuclear Structures presents an overview of features of the intranuclear environment, followed by the most recent procedures for isolating nuclei from a wide range of cell types including muscle cells, yeast, oocytes, cells with polytene nuclei, Arabidopsis, trypanosomes, and dinoflagellates. The latest methods are described for isolating and working with nucleoli, constitutive heterochromatin, pathology-associated inclusions, and chromatin and for examining glycosylation, sumoylation, and ADP-ribosylation of nuclear proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls.
The Nucleus, Volume I: Nuclei and Subnuclear Structures is an essential reference for scientists who are working on our rapidly growing understanding of the cell nucleus and its activities.
Editors and Affiliations
Bibliographic Information
Book Title: The Nucleus
Book Subtitle: Volume 1: Nuclei and Subnuclear Components
Editors: Ronald Hancock
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-406-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-977-2Published: 28 August 2008
Softcover ISBN: 978-1-61737-863-8Published: 10 December 2010
eBook ISBN: 978-1-59745-406-3Published: 26 October 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVIII, 323
Topics: Biochemistry, general, Cell Biology, Cell Physiology