Editors:
- Unique combination of reproducible laboratory protocols and state-of-the-art bioinformatics methods dedicated to functional proteomics
- A comprehensive methodological compendium, covering sample preparation, protein separation, mass-spectrometry as well as data mining, documentation and integration
- Offers methods, workflows and strategies to overcome current pitfalls and shortcomings in proteome analysis
Part of the book series: Methods in Molecular Biology (MIMB, volume 484)
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Table of contents (33 protocols)
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Front Matter
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Protein Expression Profiling
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Front Matter
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About this book
As the emerging field of proteomics continues to expand at an extremely rapid rate, the relative quantification of proteins, targeted by their function, becomes its greatest challenge. Complex analytical strategies have been designed that allow comparative analysis of large proteomes, as well as in depth detection of the core proteome or the interaction network of a given protein of interest. In Functional Proteomics: Methods and Protocols, expert researchers describe the latest protocols being developed to address the problems encountered in high-throughput proteomics projects, with emphasis on the factors governing the technical choices for given applications. The case studies within the volume focus on the following three crucial aspects of the experimental design: 1) the strategy used for the selection, purification and preparation of the sample to be analyzed by mass spectrometry, 2) the type of mass spectrometer used and the type of data to be obtained from it, and 3) the method used for the interpretation of the mass spectrometry data and the search engine used for the identification of the proteins in the different types of sequence data banks available. As a part of the highly successful Methods in Molecular Biology™ series, the chapters compile step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Functional Proteomics: Methods and Protocols is an ideal resource for all scientists pursuing this developing field and its multitudinous data.
Reviews
Editors and Affiliations
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Laboratoire de Bioinformatique et Génomique Intégratives, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
Julie D. Thompson
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Department of Protein Science Helmholtz Zentrum München, German Research Center for Environmental Health, Munich-Neuherberg, Germany
Marius Ueffing
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LSMBO, ECPM, Institut Pluridisciplinaire Hubert Curien, Strasbourg, France
Christine Schaeffer-Reiss
Bibliographic Information
Book Title: Functional Proteomics
Book Subtitle: Methods and Protocols
Editors: Julie D. Thompson, Marius Ueffing, Christine Schaeffer-Reiss
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-398-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-971-0Published: 12 August 2008
Softcover ISBN: 978-1-61737-860-7Published: 16 December 2010
eBook ISBN: 978-1-59745-398-1Published: 01 July 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVIII, 596
Number of Illustrations: 111 b/w illustrations, 4 illustrations in colour
Topics: Biochemistry, general, Protein Science, Proteomics, Mass Spectrometry