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Hepatitis C

Methods and Protocols

  • Book
  • © 2009

Overview

Editors:
  1. Hengli Tang

    1. Florida State University, Tallahassee, USA

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  • Provides the most up-to-date technical information on the new cell culture infection system as written by leading experts in the field
  • Presents both an overview and detailed protocols on the replicon system used by virtually all the HCV labs around the world
  • Covers a wide range of multi-discipline approaches adopted for HCV research, including NMR-based structure determination and mathematical modelling
  • Broadly applicable guides valuable for studying the replication of other positive-strand RNA viruses
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 510)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xxi
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  2. Detection and Quantification of HCV

    1. Front Matter

      Pages 1-1
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    2. Detection and Quantification of Serum or Plasma HCV RNA: Mini Review of Commercially Available Assays

      • Helene Le Guillou-Guillemette, Francoise Lunel-Fabiani
      Pages 3-14

    3. Simultaneous Detection of Anti-HCV Antibody and HCV Core Antigen

      • Filippo Ansaldi, Giancarlo Icardi
      Pages 15-23

    4. Immunohistochemical Detection of HCV Proteins in Liver Tissue

      • Oivier Galy, Isabelle Vincent, Philippe Chevallier, Nicole Lyandrat, Isabelle Chemin
      Pages 25-30

  3. HCV Nomenclature, Genotyping, and Quasispecies

    1. Front Matter

      Pages 31-31
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    2. Nomenclature and Numbering of the Hepatitis C Virus

      • Carla Kuiken, Peter Simmonds
      Pages 33-53

    3. A Real-Time Taqman® Method for Hepatitis C Virus Genotyping and Methods for Further Subtyping of Isolates

      • Kathryn J. Rolfe, Tim G. Wreghitt, Graeme J. M. Alexander, Martin D. Curran
      Pages 55-71

    4. Characterization of HCV Quasispecies in the Hypervariable Region 1 (HVR 1) by In Vitro Translation and Mass Spectrometry

      • Carmen Yea, Melissa Ayers, Raymond Tellier
      Pages 73-81

  4. HCV Replication in Vitro

    1. Front Matter

      Pages 83-83
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    2. Purification and Crystallization of NS5A Domain I of Hepatitis C Virus

      • Joseph Marcotrigiano, Timothy Tellinghuisen
      Pages 85-94

    3. NS5A Phosphorylation and Hyperphosphorylation

      • Petra Neddermann
      Pages 95-110

    4. Preparation and Handling of Hepatitis C Viral Proteins NS3 and NS5B for Structural Studies

      • Stefania Di Marco, Andrea Carfí
      Pages 111-124

    5. Structural and Functional Analysis of the HCV p7 Protein

      • Nathalie Saint, Roland Montserret, Christophe Chipot, François Penin
      Pages 125-143

    6. HCV Replicons: Overview and Basic Protocols

      • Volker Lohmann
      Pages 145-163

    7. Reverse Transcription PCR-Based Sequence Analysis of Hepatitis C Virus Replicon RNA

      • Timothy L. Tellinghuisen, Brett D. Lindenbach
      Pages 165-175

    8. Studying HCV RNA Synthesis In Vitro with Replication Complexes

      • Wengang Yang, Mingjun Huang
      Pages 177-184

    9. Proteomics Study of the Hepatitis C Virus Replication Complex

      • Kyungsoo Chang, Tianyi Wang, Guangxiang Luo
      Pages 185-193

    10. Investigation of the Hepatitis C Virus Replication Complex

      • Volker Brass, Rainer Gosert, Darius Moradpour
      Pages 195-209

    11. Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease

      • Kui Li
      Pages 211-226
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Keywords

About this book

When I was ?rst approached by Dr. Hengli Tang about writing the foreword to this book, the second edition of Hepatitis C Virus, I was a little puzzled: Book forewords are penned by persons far more famous than I am. Surely there were better people to write this, with a handful of the true visionaries in the ?eld immediately coming to my mind. However, after some thought and self-re?ection, I realized that I might just be the right person for the job. I remember the publication of the ?rst edition of Hepatitis C Virus in 1998, the year I began reading about hepatitis C virus with great interest. I purchased a copy of the ?rst edition from my meager graduate student stipend and read it from cover to cover, along with a variety of hepatitis C publications, to try to get a feel if this hepatitis C virus ?eld was something I might want to work on in the future. At the time, the ?rst edition was among the most comprehensive collection of hepatitis C protocols and methods available in one volume. I was amazed at the progress that had been made in hepatitis C research, and even more amazed as I learned more about the experimental challenges facing the ?eld at that time.

Reviews

From the reviews of the second edition: “This second edition contains much that was not available for the first edition; replicons, tissue culture infection systems, infectious pseudotype particles, etc. … The chapters are authored by acknowledged experts and are authoritatively written. … Of particular value are the notes sections which highlight practical pitfalls and how to avoid them. Given the size of the hepatitis C community … this book will be a success and should adorn the shelves of many laboratories.” (David J. Rowlands, Microbiology Today, July, 2009)

Editors and Affiliations

  • Florida State University, Tallahassee, USA

    Hengli Tang

Bibliographic Information

  • Book Title: Hepatitis C

  • Book Subtitle: Methods and Protocols

  • Editors: Hengli Tang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-394-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-970-3Published: 10 December 2008

  • Softcover ISBN: 978-1-61737-859-1Published: 19 November 2010

  • eBook ISBN: 978-1-59745-394-3Published: 14 November 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XXII, 466

  • Number of Illustrations: 73 b/w illustrations, 1 illustrations in colour

  • Additional Information: Originally published as volume 19 in the series: Methods Molecular Med.

  • Topics: Hepatology, Infectious Diseases, Virology

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