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Glycovirology Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Richard J. Sugrue

    1. MRC Virology Unit, Institute of Virology, Glasgow, UK

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  • An introductory overview of viruses and glycosylation
  • In-depth coverage of a variety of glycoproteins
  • Chapters written by world-renown leaders in the field

Part of the book series: Methods in Molecular Biology (MIMB, volume 379)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-x
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  2. Viruses and Glycosylation

    • Richard J. Sugrue
    Pages 1-13

  3. Interaction Between Respiratory Syncytial Virus and Glycosaminoglycans, Including Heparan Sulfate

    • Louay K. Hallak, Steven A. Kwilas, Mark E. Peeples
    Pages 15-34

  4. Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System

    • Sehaam Khan, Mah-Lee Ng, Yee-Joo Tan
    Pages 35-50

  5. The C Type Lectins DC-SIGN and L-SIGN

    • Pierre-Yves Lozach, Laura Burleigh, Isabelle Staropoli, Ali Amara
    Pages 51-68

  6. Functional Analysis of the N-Linked Glycans Within the Fusion Protein of Respiratory Syncytial Virus

    • Ping Li, Helen W. McL. Rixon, Gaie Brown, Richard J. Sugrue
    Pages 69-83

  7. Expression and Purification of Viral Glycoproteins Using Recombinant Vaccinia Viruses for Functional and Structural Studies

    • Zhu-Nan Li, David A. Steinhauer
    Pages 85-95

  8. The Use of Two-Dimensional SDS-PAGE to Analyze the Glycan Heterogeneity of the Respiratory Syncytial Virus Fusion Protein

    • Terence P. McDonald, Richard J. Sugrue
    Pages 97-108

  9. The Use of Monoclonal Antibodies and Lectins to Identify Changes in Viral Glycoproteins That are Influenced by Glycosylation

    • Joanna Rawling, José A. Melero
    Pages 109-125

  10. Expression, Glycosylation, and Modification of the Spike (S) Glycoprotein of SARS CoV

    • Shuo Shen, Timothy H. P. Tan, Yee-Joo Tan
    Pages 127-135

  11. Analysis of Glycoproteins of Viruses in the Family Bunyaviridae

    • Xiaohong Shi, Richard M. Elliott
    Pages 137-148

  12. Secretion of the Respiratory Syncytial Virus Fusion Protein From Insect Cells Using the Baculovirus Expression System

    • Boon-Huan Tan, Gaie Brown, Richard J. Sugrue
    Pages 149-161

  13. Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris

    • Boon-Huan Tan, Jian Lin Fu, Richard J. Sugrue
    Pages 163-176

  14. Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins

    • Alexander W. Tarr, Ania M. Owsianka, Alexandra Szwejk, Jonathan K. Ball, Arvind H. Patel
    Pages 177-197

  15. Back Matter

    Pages 199-205
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Keywords

About this book

Glycovirology Protocols reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins.

Editors and Affiliations

  • MRC Virology Unit, Institute of Virology, Glasgow, UK

    Richard J. Sugrue

Bibliographic Information

  • Book Title: Glycovirology Protocols

  • Editors: Richard J. Sugrue

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-393-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-590-3Published: 02 February 2007

  • Softcover ISBN: 978-1-61737-664-1Published: 19 November 2010

  • eBook ISBN: 978-1-59745-393-6Published: 18 January 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 206

  • Topics: Infectious Diseases, Virology, Immunology

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