Overview
- Completely updated edition with new chapters and topics
- In-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems
- Detailed protocols for protein production using different in vitro transcription and translation systems
Part of the book series: Methods in Molecular Biology (MIMB, volume 375)
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Table of contents (13 protocols)
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Technologies
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Applications
Keywords
About this book
A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.
Editors and Affiliations
Bibliographic Information
Book Title: In Vitro Transcription and Translation Protocols
Editors: Guido Grandi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-388-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-558-3Published: 03 May 2007
Softcover ISBN: 978-1-61737-641-2Published: 09 December 2010
eBook ISBN: 978-1-59745-388-2Published: 03 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XII, 276
Number of Illustrations: 50 b/w illustrations, 1 illustrations in colour
Topics: Biochemistry, general, Human Genetics, Cell Biology, Proteomics