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In Vitro Transcription and Translation Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Guido Grandi

    1. Biochemistry and Molecular Biology Unit, Novartis Vaccines, Siena, Italy

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  • Completely updated edition with new chapters and topics
  • In-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems
  • Detailed protocols for protein production using different in vitro transcription and translation systems

Part of the book series: Methods in Molecular Biology (MIMB, volume 375)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-xi
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  2. Technologies

    1. Front Matter

      Pages 1-1
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    2. Energy Systems for ATP Regeneration in Cell-Free Protein Synthesis Reactions

      • Kara A. Calhoun, James R. Swartz
      Pages 3-17

    3. Continuous-Exchange Protein-Synthesizing Systems

      • Vladimir A. Shirokov, Aigar Kommer, Vyacheslav A. Kolb, Alexander S. Spirin
      Pages 19-55

    4. Cell-Free Production of Integral Membrane Proteins on a Preparative Scale

      • Christian Klammt, Daniel Schwarz, Volker Dötsch, Frank Bernhard
      Pages 57-78

    5. SIMPLEX: Single-Molecule PCR-Linked In Vitro Expression

      • Suang Rungpragayphan, Tsuneo Yamane, Hideo Nakano
      Pages 79-94

    6. Methods for High-Throughput Materialization of Genetic Information Based on Wheat Germ Cell-Free Expression System

      • Tatsuya Sawasaki, Ryo Morishita, Mudeppa D. Gouda, Yaeta Endo
      Pages 95-106

    7. Exogenous Protein Expression in Xenopus Oocytes

      • Elena Bossi, Maria Serena Fabbrini, Aldo Ceriotti
      Pages 107-131

  3. Applications

    1. Front Matter

      Pages 133-133
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    2. In Vitro Translation to Study HIV Protease Activity

      • Zene Matsuda, Mutsunori Iga, Kosuke Miyauchi, Jun Komano, Kazuhiro Morishita, Akihiko Okayama et al.
      Pages 135-149

    3. The Protein Truncation Test in Mutation Detection and Molecular Diagnosis

      • Oliver Hauss, Oliver Müller
      Pages 151-164

    4. Creation of Novel Enantioselective Lipases by SIMPLEX

      • Yuichi Koga, Tsuneo Yamane, Hideo Nakano
      Pages 165-181

    5. In Vitro Transcription and Translation Coupled to Two-Dimensional Electrophoresis for Bacterial Proteome Analysis

      • Nathalie Norais, Ignazio Garaguso, Germano Ferrari, Guido Grandi
      Pages 183-209

    6. In Vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation, and Immunoprecipitation Analysis

      • Orit Gat, Haim Grosfeld, Avigdor Shafferman
      Pages 211-233

    7. Functional Expression of Type 1 Rat GABA Transporter in Microinjected Xenopus laevis Oocytes

      • Stefano Giovannardi, Andrea Soragna, Simona Magagnin, Laura Faravelli
      Pages 235-255

    8. Production of Protein for Nuclear Magnetic Resonance Study Using the Wheat Germ Cell-Free System

      • Toshiyuki Kohno, Yaeta Endo
      Pages 257-272

  4. Back Matter

    Pages 273-276
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Keywords

About this book

A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.

Editors and Affiliations

  • Biochemistry and Molecular Biology Unit, Novartis Vaccines, Siena, Italy

    Guido Grandi

Bibliographic Information

  • Book Title: In Vitro Transcription and Translation Protocols

  • Editors: Guido Grandi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-388-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-558-3Published: 03 May 2007

  • Softcover ISBN: 978-1-61737-641-2Published: 09 December 2010

  • eBook ISBN: 978-1-59745-388-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 276

  • Number of Illustrations: 50 b/w illustrations, 1 illustrations in colour

  • Topics: Biochemistry, general, Human Genetics, Cell Biology, Proteomics

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