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Pyrosequencing Protocols

  • Book
  • © 2007

Overview

Part of the book series: Methods in Molecular Biology (MIMB, volume 373)

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Table of contents (14 protocols)

Keywords

About this book

Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method.

Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.

Editors and Affiliations

  • Division of Oncology, Washington University School of Medicine, St. Louis

    Sharon Marsh

Bibliographic Information

  • Book Title: Pyrosequencing Protocols

  • Editors: John M. Walker, Sharon Marsh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597453773

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-645-0Published: 21 November 2006

  • Softcover ISBN: 978-1-61737-693-1Published: 01 December 2010

  • eBook ISBN: 978-1-59745-377-6Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 196

  • Topics: Biochemistry, general, Human Genetics

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