Skip to main content
  • Book
  • © 2008

Allergy Methods and Protocols

  • Aims to assist the researcher in gaining insight into the molecular mechanisms involved in allergy by featuring an array of protocols
  • The protocols cover a range of disciplines

Part of the book series: Methods in Molecular Medicine (MIMM, volume 138)

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (27 protocols)

  1. Front Matter

    Pages i-xi
  2. T Cell — Primary Culture from Peripheral Blood

    • Monika Raulf-Heimsoth
    Pages 17-30
  3. Production of T-Cell Lines

    • Helga Kahlert
    Pages 31-41
  4. Production of Human T-Cell Clones

    • Adrienne Verhoef
    Pages 43-50
  5. Mapping of Human T-Cell Epitopes of Allergens

    • Thomas Zeiler, Tuomas Virtanen
    Pages 51-56
  6. Ultrasensitive ELISA for Measurement of Human Cytokine Responses in Primary Culture

    • William P. Stefura, J. Darren Campbell, Renée Douville, Monique J. Stinson, F. Estelle Simons, Allan B. Becker et al.
    Pages 107-119
  7. Quantification of Human Chemokine Production in TLR-Stimulated and Antigen-Specific Recall Responses

    • Monique Stinson, Renee Douville, Yuriy Lissitsyn, Melanie Blanchard, William Stefura, Estelle Simons et al.
    Pages 121-131
  8. Standardization of Allergen Extracts

    • Jørgen Nedergaard Larsen, Sten Dreborg
    Pages 133-145
  9. Immunoelectrophoresis for the Characterization of Allergen Extracts

    • Gitte Nordskov Hansen, Jørgen Nedergaard Larsen
    Pages 147-165
  10. Conjugation of Haptens

    • Ranulfo Lemus, Meryl H. Karol
    Pages 167-182
  11. Monoclonal Antibodies

    • Helga Kahlert, Oliver Cromwell
    Pages 183-196
  12. Purification of Antibodies

    • Per H. Larsson
    Pages 197-207
  13. Assay of Air Sample Eluates

    • Anne Renström, Susan Gordon
    Pages 217-225
  14. The Halogen Assay - A New Technique for Measuring Airborne Allergen

    • Euan Tovey, Sandra De Lucca, Leanne Poulos, Tim O’Meara
    Pages 227-246

About this book

In recent years, allergy research has focused on the causes and mechanisms of allergy. In parallel, there is also an impetus to try to understand mechanisms of natural tolerance and immunotherapy where allergy is being dampened. In Allergy: Methods and Protocols a groundbreaking new title from the Methods in Molecular Medicine series, leaders in the field provide assistance for researchers to gain insight into the molecular mechanisms involved in allergy by featuring an array of protocols. These cover a range of disciplines including allergy, immunology, cell biology and histology and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, T regulatory cells. Techniques discussed include; B and T cell epitope mapping, characterization of allergens, conjugation of haptens, preparation of monoclonal antibodies, collection and sampling of airborne allergens, IgG antibodies and facilitated antigen blocking assays, identification and purification of mast cells and in situ hybridisation. Allergy: Methods and Protocols will be a remarkably useful bench tool for anyone embarking in or continuing with their research in allergy.

Reviews

From the reviews:

"The purpose is to provide a comprehensive and readily accessible guide to techniques used in the investigation of allergic diseases. ... Students embarking on an investigative career in allergy and immunology will find this book particularly useful. ... would find a ready place in most laboratories where postgraduate students are working. ... It can be highly recommended to researchers who are starting out, and to those who would like an authoritative and succinct background to their work." (Ronald S. Walls, Doody's Review Service, October, 2008)

Editors and Affiliations

  • Department of Occupational and Environmental Medicine, Imperial College, London, UK

    Meinir G. Jones

  • St. George’s University of London, London, UK

    Penny Lympany

Bibliographic Information

  • Book Title: Allergy Methods and Protocols

  • Editors: Meinir G. Jones, Penny Lympany

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1007/978-1-59745-366-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-0-89603-896-7Published: 04 April 2008

  • Softcover ISBN: 978-1-61737-243-8Published: 09 November 2010

  • eBook ISBN: 978-1-59745-366-0Published: 09 July 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XI, 352

  • Number of Illustrations: 39 b/w illustrations, 1 illustrations in colour

  • Topics: Immunology, Laboratory Medicine

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access