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Mitochondria

Practical Protocols

  • Book
  • © 2007

Overview

Editors:
  1. Dario Leister

    1. Department für Biologie I, Botanik, Ludwig-Maximilians-Universität München, München, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Johannes M. Herrmann

    1. Zellbiologie, Universität Kaiserslautern, Kaiserslautern, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Comprehensive lab manual for mitochondrial research
  • Introduction to different model organisms for studying mitochondrial function
  • Fusion and fission of mitochondria
  • Biogenesis of mitochondria
  • Detailed overview of functional genomic approaches for studying mitochondrial function

Part of the book series: Methods in Molecular Biology (MIMB, volume 372)

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Table of contents (39 protocols)

  1. Front Matter

    Pages i-xvi
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  2. Model Systems to Study Mitochondrial Functions

    1. Front Matter

      Pages 1-1
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    2. The Mitochondria of Cultured Mammalian Cells

      • Florence Malka, Karine Auré, Steffi Goffart, Johannes N. Spelbrink, Manuel Rojo
      Pages 3-16

    3. The Mitochondria of Cultured Mammalian Cells

      • Steffi Goffart, Peter Martinsson, Florence Malka, Manuel Rojo, Johannes N. Spelbrink
      Pages 17-32

    4. Drosophila melanogaster as a Model System to Study Mitochondrial Biology

      • Miguel Angel Fernández-Moreno, Carol L. Farr, Laurie S. Kaguni, Rafael Garesse
      Pages 33-49

    5. Isolation and Functional Analysis of Mitochondria From the Nematode Caenorhabditis elegans

      • Leslie I. Grad, Leanne C. Sayles, Bernard D. Lemire
      Pages 51-66

    6. Isolation of Mitochondria From Procyclic Trypanosoma brucei

      • André Schneider, Fabien Charrière, Mascha Pusnik, Elke K. Horn
      Pages 67-80

    7. Saccharomyces cerevisiae as a Model Organism to Study Mitochondrial Biology

      • Katrin Altmann, Mark Dürr, Benedikt Westermann
      Pages 81-90

    8. Studying Mitochondria in an Attractive Model: Schizosaccharomyces pombe

      • Stéphane Chiron, Mauricette Gaisne, Emmanuelle Guillou, Pascale Belenguer, G. Desmond Clark-Walker, Nathalie Bonnefoy
      Pages 91-105

    9. Neurospora crassa as a Model Organism for Mitochondrial Biogenesis

      • Frank E. Nargang, Doron Rapaport
      Pages 107-123

    10. Isolation of Intact, Functional Mitochondria From the Model Plant Arabidopsis thaliana

      • Lee J. Sweetlove, Nicolas L. Taylor, Christopher J. Leaver
      Pages 125-136

    11. Chlamydomonas reinhardtii: The Model of Choice to Study Mitochondria From Unicellular Photosynthetic Organisms

      • Soledad Funes, Lars-Gunnar Franzén, Diego González-Halphen
      Pages 137-149

  3. Genetics and Molecular Biology

    1. Front Matter

      Pages 151-151
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    2. Directed Alteration of Saccharomyces cerevisiae Mitochondrial DNA by Biolistic Transformation and Homologous Recombination

      • Nathalie Bonnefoy, Thomas D. Fox
      Pages 153-166

    3. Screens for Mitochondrial Mutants in Yeast

      • Françoise Foury
      Pages 167-176

    4. Transcript End Mapping and Analysis of RNA Editing in Plant Mitochondria

      • Frank Kempken, Nina Bolle, Joachim Forner, Stefan Binder
      Pages 177-192

    5. In Vitro Analysis of the Yeast Mitochondrial RNA Polymerase

      • Elizabeth A. Amiott, Judith A. Jaehning
      Pages 193-206

    6. Functional Analysis by Inducible RNA Interference in Drosophila melanogaster

      • Yuichi Matsushima, Cristina Adán, Rafael Garesse, Laurie S. Kaguni
      Pages 207-217

    7. Analysis of Replicating Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis

      • Aurelio Reyes, Takehiro Yasukawa, Ian J. Holt
      Pages 219-232

  4. Biochemistry

    1. Front Matter

      Pages 233-233
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Keywords

About this book

Mitochondria: Practical Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike.

Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis.

Editors and Affiliations

  • Department für Biologie I, Botanik, Ludwig-Maximilians-Universität München, München, Germany

    Dario Leister

  • Zellbiologie, Universität Kaiserslautern, Kaiserslautern, Germany

    Johannes M. Herrmann

Bibliographic Information

  • Book Title: Mitochondria

  • Book Subtitle: Practical Protocols

  • Editors: Dario Leister, Johannes M. Herrmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-365-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-667-2Published: 12 June 2007

  • Softcover ISBN: 978-1-62703-833-1Published: 26 November 2014

  • eBook ISBN: 978-1-59745-365-3Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 586

  • Topics: Cell Biology, Human Genetics

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