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  • © 2007

Biological Aging

Methods and Protocols

  • New and novel techniques in biological aging research
  • Cutting-edge applications of proteomics and metabolomics
  • Identifying genes that extend lifespan using high-throughput screening systems

Part of the book series: Methods in Molecular Biology (MIMB, volume 371)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xv
  2. Techniques for Analysis of Biological Aging

    • Trygve O. Tollefsbol
    Pages 1-7
  3. Aging Cell Culture

    • Sharla M. O. Phipps, Joel B. Berletch, Lucy G. Andrews, Trygve O. Tollefsbol
    Pages 9-19
  4. Methods to Detect Biomarkers of Cellular Senescence

    • Koji Itahana, Judith Campisi, Goberdhan P. Dimri
    Pages 21-31
  5. Methods for Cell Sorting of Young and Senescent Cells

    • João F. Passos, Thomas von Zglinicki
    Pages 33-44
  6. Telomere Length Analysis

    • Andrés Canela, Peter Klatt, María A. Blasco
    Pages 45-72
  7. A Method to Detect DNA Methyltransferase I Gene Transcription In Vitro in Aging Systems

    • Joel B. Berletch, Lucy G. Andrews, Trygve O. Tollefsbol
    Pages 73-80
  8. A Method to Study the Expression of DNA Methyltransferases in Aging Systems In Vitro

    • Joel B. Berletch, Sharla M. O. Phipps, Sabrina L. Walthall, Lucy G. Andrews, Trygve O. Tollefsbol
    Pages 81-87
  9. The Chronological Life Span of Saccharomyces cerevisiae

    • Paola Fabrizio, Valter D. Longo
    Pages 89-95
  10. Caloric Restriction and Life Span Determination of Yeast Cells

    • Oliver Medvedik, David A. Sinclair
    Pages 97-109
  11. Methods for Nutrigenomics and Longevity Studies in Drosophila

    • Jiatao Ye, Xiangqin Cui, Ann Loraine, Kerry Bynum, Nam Chul Kim, Gregory White et al.
    Pages 111-141
  12. Caloric Restriction Mimetics

    • Mark A. Lane, George S. Roth, Donald K. Ingram
    Pages 143-149
  13. Extension of Cell Life Span Using Exogenous Telomerase

    • Mo K. Kang, No-Hee Park
    Pages 151-165
  14. Inducing Cellular Senescence Using Defined Genetic Elements

    • Hiroshi Nakagawa, Oliver G. Opitz
    Pages 167-178
  15. Methods of Cellular Senescence Induction Using Oxidative Stress

    • Jian-Hua Chen, Susan E. Ozanne, C. Nicholas Hales
    Pages 179-189
  16. Nuclear Transfer Methods to Study Aging

    • Lin Liu, David L. Keefe
    Pages 191-207
  17. Use of Ribozymes in Cellular Aging Research

    • Custer C. Deocaris, Sunil C. Kaul, Renu Wadhwa
    Pages 209-226
  18. Methods of Evaluating the Effect of Pharmacological Drugs On Aging and Life Span in Mice

    • Vladimir N. Anisimov, Irina G. Popovich, Mark A. Zabezhinski
    Pages 227-236

About this book

Biological Aging: Methods and Protocols investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. The editors have carefully selected only those topics that are considered mainstays of the field or are showing promise in revolutionizing this relatively new science. The three main areas of focus in this cutting-edge compendium of biological aging research are: methods that are basic to understanding the fundamental mechanisms of cellular aging; techniques used to intervene in the aging process; and approaches to analyzing the many molecular processes of biological aging. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions in the exciting and expanding field of biological aging.

Reviews

From the reviews:

"For research gerontologists, this book is a boon." -Doody's Book Review, Weighted Numerical Score:90 - 4 Stars

"This can be a useful book for biogerontologists. … Comprised of 25 generally well-written articles … this book covers various methods and protocols for studying cellular aging of human cells, yeast cells, and mice. … a welcome source of useful information for researchers intending to find their way into descriptive, analytical and interventive research in biogerontology." (Suresh Rattan, Biogerontology, Issue 9, 2008)

Editors and Affiliations

  • Department of Biology, University of Alabama at Birmingham, Birmingham

    Trygve O. Tollefsbol

Bibliographic Information

  • Book Title: Biological Aging

  • Book Subtitle: Methods and Protocols

  • Editors: Trygve O. Tollefsbol

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-361-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-658-0Published: 05 April 2007

  • Softcover ISBN: 978-1-62703-906-2Published: 27 November 2014

  • eBook ISBN: 978-1-59745-361-5Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 414

  • Topics: Developmental Biology, Geriatrics/Gerontology, Cell Biology, Human Genetics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access