Single Cell Diagnostics

1. Front Matter

   Pages i-xi

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2. Noninvasive Metabolic Assessment of Single Cells

   • David K. Gardner

   Pages 1-9

3. Laser Capture Microdissection for Analysis of Single Cells

   • Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, Ricardo V. Lloyd

   Pages 11-18

4. Fluorescence In Situ Hybridization on Single Cells

   • Paul N. Scriven, Caroline Mackie Ogilvie

   Pages 19-30

5. Single Cell Polymerase Chain Reaction for Preimplantation Genetic Diagnosis

   • Karen Sermon, Martine De Rycke

   Pages 31-42

6. Real-Time Quantitative Polymerase Chain Reaction Measurement of Male Fetal DNA in Maternal Plasma

   • Bernhard Zimmermann, Xiao Yan Zhong, Wolfgang Holzgreve, Sinuhe Hahn

   Pages 43-49

7. Cell-Free Fetal DNA Plasma Extraction and Real-Time Polymerase Chain Reaction Quantification

   • Jill L. Maron, Kirby L. Johnson, Diana W. Bianchi

   Pages 51-63

8. Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies

   • Kenneth E. Pierce, Lawrence J. Wangh

   Pages 65-85

9. Efficient Isothermal Amplification of the Entire Genome from Single Cells

   • Karen V. Schowalter, Jolene Fredrickson, Alan R. Thornhill

   Pages 87-99

10. Comparative Genomic Hybridization on Single Cells

    • Lucille Voullaire, Leeanda Wilton

    Pages 101-115

11. Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells

    • James Adjaye

    Pages 117-124

12. Use of Real-Time Polymerase Chain Reaction to Measure Gene Expression in Single Cells

    • Dagan Wells

    Pages 125-133

13. Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridization

    • Dong Gui Hu, Xin Yuan Guan, Nicole Hussey

    Pages 135-151

14. Microarray Technology for Mutation Analysis of Low-Template DNA Samples

    • Chelsea Salvado, David Cram

    Pages 153-173

15. Back Matter

    Pages 175-182

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The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.

• DNA
• FISH
• Fluoreszenz in situ-Hybridisierung
• Microarray
• Mutation
• PCR
• Quantitative PCR
• Termination
• chromosome
• gene expression
• genes
• hybridization

From the reviews:

"Single cell diagnostics has become an increasingly important field for both clinicians and researchers who are involved in infertility treatment. … For researchers this book provides not only detailed state of the art protocols for single cell diagnostics, but also valuable notes on troubleshooting and pitfalls. For clinicians in the field of infertility this nice overview gives an insight into single cell diagnostics and its scientific background. … This book can be warmly recommended to clinicians and researchers caring for patients with fertility problems." (G. Haidl and J. P. Allam, Andrologia, Vol. 40 (1), 2008)

"This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. … The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. … The chapters are written by experts and the book is carefully edited. … The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics." (Bruce A. Fenderson, Doody’s Review Service, October, 2007)

• The London Bridge Fertility, Gynecology and Genetics Centre, London, UK

  Alan Thornhill

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