Overview
- Editors:
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Raymond Tyther
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David Sheehan
- Covers a wide biological range of proteome sources including microbes, plants and animals
- Addresses key aspects of experimental design
- Includes some of the latest approaches such as DIGE and detection of post-translational modification
- Includes supplementary material: sn.pub/extras
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Table of contents (36 protocols)
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- Mireille Starita-Geribaldi
Pages 31-45
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- Ben Herbert, Elizabeth Harry
Pages 47-63
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- Matthias Plöscher, Bernhard Granvogl, Veronika Reisinger, Axel Masanek, Lutz Andreas Eichacker
Pages 65-82
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- Alexander Leitner, Wolfgang Lindner
Pages 83-101
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- Bettina Levänen, Åsa M. Wheelock
Pages 112-129
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- Pan-Young Jeong, Keun Na, Mi-Jeong Jeong, David Chitwood, Yhong-Hee Shim, Young-Ki Paik
Pages 145-169
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- Claus Zabel, Joachim Klose
Pages 171-196
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- Suze Chora, Maria Bebianno, Michèle Roméo
Pages 197-204
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- Anne von Zychlinski, Wilhelm Gruissem
Pages 205-220
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- Katrin Marcus, Cornelia Joppich, Caroline May, Kathy Pfeiffer, Barbara Sitek, Helmut Meyer et al.
Pages 221-240
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- Kelly Andringa, Adrienne King, Shannon Bailey
Pages 241-258
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- Kiichi Fukui, Susumu Uchiyama
Pages 259-271
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- Diana M. Wong, Khosrow Adeli
Pages 273-289
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- Won-A Joo, David Speicher
Pages 291-304
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About this book
The human genome and other large-scale genome sequencing projects have inevitably led to a focus on the proteins encoded by genes. The field of proteomics has grown enormously as a result and a number of high-throughput technologies have now been developed allowing discovery-led investigations of protein populations rather than more traditional hypothesis-led studies on single proteins. These high-throughput techno- gies include gene and protein microarrays, the yeast two-hybrid system, and various mass spectrometry methodologies. However, despite developments and improvements in these technologies, two-dimensional electrophoresis (2DE) remains one of the most widely used approaches. This technique was revolutionised by the development of immobilised pH gradient strips which are now commercially available. This has made possible highly reproducible separations of matched samples. Developments in staining, mass spectr- etry, and bioinformatics supported these developments and have led to a measure of standardisation in design, execution, and analysis of proteomics experiments. This book began life as a proposed update of the excellent volume 2DE Protocols edited by Andrew Link of the University of Washington at Seattle. However, we re- ised that 2DE has undergone major development in aspects of its technology in recent years and we were anxious to reflect these in the present volume. We are also conscious that many researchers have now begun to apply proteomics methodologies to a growing range of biological material and we were anxious to include contributions to reflect the challenges posed in sample preparation in less widely used organisms.
