Cancer Stem Cells

1. Front Matter

   Pages i-xv

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2. Defining Cancer Stem Cells by Xenotransplantation in Zebrafish

   • Michael C. Dovey, Leonard I. Zon

   Pages 1-5

3. Analysis of Cancer Stem Cell Metastasis in Xenograft Animal Models

   • Yibin Kang

   Pages 7-19

4. Identification of Murine and Human Acute Myeloid Leukemia Stem Cells

   • Aniruddha J. Deshpande, Farid Ahmed, Christian Buske

   Pages 21-35

5. Methods for Analysis of Brain Tumor Stem Cell and Neural Stem Cell Self-Renewal

   • Ichiro Nakano, Harley I. Kornblum

   Pages 37-56

6. Prospective Identification of Cancer Stem Cells with the Surface Antigen CD133

   • Kyeung Min Joo, Do-Hyun Nam

   Pages 57-71

7. Neurosphere Culture and Human Organotypic Model to Evaluate Brain Tumor Stem Cells

   • Hugo Guerrero-Cázares, Kaisorn L. Chaichana, Alfredo Quiñones-Hinojosa

   Pages 73-83

8. Methodologies in Assaying Prostate Cancer Stem Cells

   • Hangwen Li, Ming Jiang, Sofia Honorio, Lubna Patrawala, Collene R. Jeter, Tammy Calhoun-Davis et al.

   Pages 85-138

9. Characterization of Nonmalignant and Malignant Prostatic Stem/Progenitor Cells by Hoechst Side Population Method

   • Murielle Mimeault, Surinder K. Batra

   Pages 139-149

10. Anchorage-Independent Growth of Prostate Cancer Stem Cells

    • Shunyou Wang

    Pages 151-160

11. Identification of Human Pancreatic Cancer Stem Cells

    • Chenwei Li, C. J. Lee, Diane M. Simeone

    Pages 161-173

12. Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma

    • Laurie Ailles, Mark Prince

    Pages 175-193

13. Pituitary Adenoma Stem Cells

    • Patrizia Tunici, John S. Yu

    Pages 195-201

14. Genome-Wide DNA Methylation Profiling: The mDIP-Chip Technology

    • Yin Shen, Shaun D. Fouse, Guoping Fan

    Pages 203-216

15. The Contribution of Niche-Derived Factors to the Regulation of Cancer Cells

    • Julie B. Sneddon

    Pages 217-232

16. Dendritic Cell Vaccines for Cancer Stem Cells

    • Serena Pellegatta, Gaetano Finocchiaro

    Pages 233-247

17. Maintaining and Engineering Neural Stem Cells for Delivery of Genetically Encoded Therapy to Brain Tumors

    • Jennifer Katz, Joseph Krueger, Brunhilde Felding-Habermann, Evan Y. Snyder

    Pages 249-259

18. Measurement of Multiple Drug Resistance Transporter Activity in Putative Cancer Stem/Progenitor Cells

    • Vera S. Donnenberg, E. Michael Meyer, Albert D. Donnenberg

    Pages 261-279

19. Back Matter

    Pages 281-283

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Cancer is a devastating disease that affects millions of people in the world. Our the- pies for tumors have mostly been based on classical chemotherapy and antiproliferative treatments. More recently, directed therapies against a causative oncogene have led to prominent reduction in tumor rates for some cancers. For instance, chronic myelogenous leukemia that is due to a BCR–ABL translocation can be targeted with Gleevec. Despite these advances in individual tumors, a number of patients are treated for their primary tumors and ultimately relapse. Relapsing tumors can be due to resistance to chemotherapy or to antioncogene drugs. Another hypothesis is that heterogeneity in the tumor leads to an inability of classical chemotherapy to completely eradicate all cells of the tumor. This concept of heterogeneity has led some investigators to propose a cancer stem cell model. As patients are treated with chemotherapy, most of the dividing cells are killed, but this leaves a small subset of cells that have the ability to remake the entire tumor. These are cancer stem cells. They possess the signals of self-renewal and yet can also differentiate. If one could understand more about the cells that remain after classical chemotherapy or the cells that can remake the tumor among the heterogeneous population, this information would have a huge impact on our treatment of cancer.

• Brain cancer
• Carcinom
• DNA
• Head and neck cancer
• Leukemia
• Marker expression
• Neurospheres
• Pancreatic cancer
• Pituitary adenoma
• Prostate cancer
• Tumors
• molecular biology
• Cell culture

From the reviews:

“This volume in the popular Methods in Molecular Biology series provides a state-of-the art compendium of research techniques and protocols for identifying and culturing rare populations of cancer stem cells. … The audience includes basic science and clinical researchers who are interested in stem cell biology as well as mechanisms of carcinogenesis and malignancy. The book also will be valued by biomedical scientists and clinicians interested in clinical oncology. … The topic is fascinating and clinically relevant.” (Bruce A. Fenderson, Doody’s Review Service, June, 2010)

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