Overview
- Cutting-edge techniques for introducing isotopic labels
- Affinity enrichment of phosphopeptides coupled with stable-isotope labeling
- Tandem MS for disease detection
- Procedures for characterization of the phosphoproteome
- Quantification of proteins by MS without isotopic labels
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 359)
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Table of contents (14 protocols)
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Front Matter
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Back Matter
Keywords
About this book
Quantitative Proteomics by Mass Spectrometry, from the Methods in Molecular Biology™ series, is a compendium of cutting-edge protocols for quantitative proteomics, and presents the most significant methods used in the field today. The focus on mass spectrometry (MS) is integral, as MS has, and will continue to be, an essential tool in proteomics for studying complex biological systems and human diseases. This volume, written and compiled by leading quantitative proteomic experts, is an indispensable resource in the search for novel biomarkers.
Quantitative Proteomics by Mass Spectrometry presents several innovative MS quantitative procedures, including a variety of methods for introducing isotopic labels and quantifying post-translational modifications. Some of these methods include growing an organism in isotope-enriched media, performing trypsin proteolysis in the presence of 18O-water, reacting protein samples with isotopically labeled reagents, quantifying relative amount of proteins without the use of any isotopic labels. Attention is also given to state-of-the-art techniques for the characterization of the phosphoproteome and tandem MS for detection of inborn errors of metabolism. Specifically, the procedure for determinations of enzymatic activity could be used for large-scale screening of newborns. The protocols in this volume expand both the breadth and depth of readily available methods for quantitative proteomic researchers using MS..
Reviews
From the reviews:
“Quantitative Proteomics by Mass Spectrometry describes protocols of various quantitative proteomics procedures utilizing mass spectrometry. … The publication of this book is timely. … provides useful information regarding sample preparation/handling, reagent preparation/handling, mass spectrometry analysis etc. for successful experiments. … In summary, the book serves as an excellent guide for both the experienced investigator and novice in the field who is contemplating using quantitative proteomics by mass spectrometry.” (Masaru Miyagi, Journal of the American Society for Mass Spectrometry, Vol. 18, 2007)Editors and Affiliations
Bibliographic Information
Book Title: Quantitative Proteomics by Mass Spectrometry
Editors: Salvatore Sechi
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-255-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2007
Hardcover ISBN: 978-1-58829-571-2Published: 05 February 2007
Softcover ISBN: 978-1-61737-648-1Published: 09 December 2010
eBook ISBN: 978-1-59745-255-7Published: 05 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 218
Number of Illustrations: 48 b/w illustrations, 4 illustrations in colour
Topics: Biochemistry, general, Cell Biology, Proteomics, Biomedicine general