Plant Proteomics

1. Front Matter

   Pages i-xiii

   PDF

2. Total Protein Extraction with TCA-Acetone

   • Valérie Méchin, Catherine Damerval, Michel Zivy

   Pages 1-8

3. Phenol Extraction of Proteins for Proteomic Studies of Recalcitrant Plant Tissues

   • Mireille Faurobert, Esther Pelpoir, Jamila Chaïb

   Pages 9-14

4. Protein Extraction from Cereal Seeds

   • Gérard Branlard, Emmanuelle Bancel

   Pages 15-25

5. Protein Extraction from Xylem and Phloem Sap

   • Julia Kehr, Martijn Rep

   Pages 27-35

6. Protein Extraction from Woody Plants

   • Christophe Plomion, Céline Lalanne

   Pages 37-41

7. Isolation of Chloroplast Proteins from Arabidopsis thaliana for Proteome Analysis

   • Klaas J. van Wijk, Jean-Benoit Peltier, Lisa Giacomelli

   Pages 43-48

8. Isolation and Subfractionation of Plant Mitochondria for Proteomic Analysis

   • Holger Eubel, Joshua L. Heazlewood, A. Harvey Millar

   Pages 49-62

9. Extraction of Nuclear Proteins from Root Meristematic Cells

   • Fernando González-Camacho, Francisco Javier Medina

   Pages 63-72

10. Extraction of Nuclear Proteins

    • Setsuko Komatsu

    Pages 73-77

11. Isolation of Cell Wall Proteins from Medicago sativa Stems

    • Bonnie S. Watson, Lloyd W. Sumner

    Pages 79-92

12. Plant Plasma Membrane Protein Extraction and Solubilization for Proteomic Analysis

    • Véronique Santoni

    Pages 93-109

13. Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis

    • Thierry Rabilloud, Sylvie Luche, Véronique Santoni, Mireille Chevallet

    Pages 111-119

14. Two-Dimensional Electrophoresis for Plant Proteomics

    • Walter Weiss, Angelika Görg

    Pages 121-143

15. Visible and Fluorescent Staining of Two-Dimensional Gels

    • François Chevalier, Valérie Rofidal, Michel Rossignol

    Pages 145-156

16. Two-Dimensional Differential In-Gel Electrophoresis (DIGE) of Leaf and Roots of Lycopersicon esculentum

    • Matthew Keeler, Jessica Letarte, Emily Hattrup, Fatimah Hickman, Paul A. Haynes

    Pages 157-174

17. Quantitative Analysis of 2D Gels

    • Michel Zivy

    Pages 175-194

18. Multivariate Data Analysis of Proteome Data

    • Kåre Engkilde, Susanne Jacobsen, Ib Søndergaard

    Pages 195-210

19. Edman Sequencing of Proteins from 2D Gels

    • Setsuko Komatsu

    Pages 211-217

20. Peptide Mass Fingerprinting

    • Nicolas Sommerer, Delphine Centeno, Michel Rossignol

    Pages 219-234

The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22).

• Microarray
• Polyacrylamid-Gelelektrophorese
• Proteomics
• Seed
• Translation
• arabidopsis thaliana
• membrane proteins
• plasma membrane
• post-translational modification
• protein complexes
• roots

• UMR de Génétique Végétale, La Ferme du Moulon, Gif-sur-Yvette, France

  Hervé Thiellement, Michel Zivy, Catherine Damerval

• UMR de Chimie Biologique, Thiverval Grignon, France

  Valérie Méchin

Policies and ethics