High Content Screening

1. Front Matter

   Pages i-xiii

   PDF

2. Introduction to High Content Screening

   1. Front Matter

      Pages 1-1

      PDF

   2. Past, Present, and Future of High Content Screening and the Field of Cellomics

      • D. Lansing Taylor

      Pages 3-18

   3. A Pharmaceutical Company User’s Perspective on the Potential of High Content Screening in Drug Discovery

      • Ann F. Hoffman, Ralph J. Garippa

      Pages 19-31

   4. Linking Microscopy and High Content Screening in Large-Scale Biomedical Research

      • James G. Evans, Paul Matsudaira

      Pages 33-38

3. Instrumentation, Biological Application Software, and Sample Preparation

   1. Front Matter

      Pages 39-39

      PDF

   2. Requirements, Features, and Performance of High Content Screening Platforms

      • Albert H. Gough, Paul A. Johnston

      Pages 41-61

   3. Characteristics and Value of Directed Algorithms in High Content Screening

      • Richik N. Ghosh, Oleg Lapets, Jeffrey R. Haskins

      Pages 63-81

   4. Characteristics and Value of Machine Learning for Imaging in High Content Screening

      • Juergen A. Klenk

      Pages 83-94

   5. Tools for Quantitative and Validated Measurements of Cells

      • Anne L. Plant, John T. Elliott, Alessandro Tona, Dennis McDaniel, Kurt J. Langenbach

      Pages 95-107

   6. Automated Cell Plating and Sample Treatments for Fixed Cells in High Content Assays

      • Gillian R. Richards, Julie E. Kerby, Grace K. Y. Chan, Peter B. Simpson

      Pages 109-119

   7. Differentiating Primary Human Cells in Rapid-Throughput Discovery Applications

      • Daniel R. Marshak, Dale E. Greenwalt

      Pages 121-128

   8. Use of the CellCard™ System for Analyzing Multiple Cell Types in Parallel

      • Oren Beske, Daniel Bassoni, Simon Goldbard

      Pages 129-138

4. Reagents

   1. Front Matter

      Pages 139-139

      PDF

   2. Reagents to Measure and Manipulate Cell Functions

      • Kenneth A. Giuliano, D. Lansing Taylor, Alan S. Waggoner

      Pages 141-163

   3. Fluorescent Proteins and Engineered Cell Lines

      • Nick Thomas

      Pages 165-187

   4. Optimizing the Integration of Immunoreagents and Fluorescent Probes for Multiplexed High Content Screening Assays

      • Kenneth A. Giuliano

      Pages 189-193

   5. The HaloTag™

      • Georgyi V. Los, Keith Wood

      Pages 195-208

   6. Protein Labeling With FlAsH and ReAsH

      • Thomas Machleidt, Matt Robers, George T. Hanson

      Pages 209-220

   7. Exploiting Network Biology to Improve Drug Discovery

      • Marnie L. MacDonald, John K. Westwick

      Pages 221-232

There has always been some tension between proponents of hypothesis-driven and discovery-driven research in the broad field of life sciences. Academic research has been primarily focused on hypothesis-driven research. However, the success of the human genome project, a discovery-driven research approach, has opened the door to adding other types of discovery-driven research to a continuum of research approaches. In contrast, drug discovery research in the pharmaceutical industry has embraced discovery-driven research for many years. A good example has been the discovery of active compounds from large chemical libraries, through screening campaigns. The success of the human genome project has also demonstrated the need for both academic researchers and industrial researchers to now understand the functions of genes and gene products. The cell is the basic unit of life and it has been at the cellular level where function can be demonstrated most cost-effectively and rapidly. High content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell.

• bioinformatics
• biology
• cell biology
• cell lines
• genes
• proteins
• siRNA

"...very useful."  -Weighted Numberical Score: 97 - 5  Stars!-Doody's Health Science Book Review Journal

• Cellumen, Inc., Pittsburgh

  D. Lansing Taylor, Kenneth A. Giuliano

• Cellomics, Inc., Pittsburgh

  Jeffrey R. Haskins

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