Cardiovascular Proteomics

1. Front Matter

   Pages i-xv

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2. Analysis of the Heart Proteome

   1. Front Matter

      Pages 1-1

      PDF

   2. Two-Dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics

      • Michael J. Dunn

      Pages 3-13

   3. Analyzing the Cardiac Muscle Proteome by Liquid Chromatography-Mass Spectrometry-Based Expression Proteomics

      • Anthony O. Gramolini, Thomas Kislinger, Peter Liu, David H. MacLennan, Andrew Emili

      Pages 15-31

   4. Two-Dimensional Differential Gel Electrophoresis of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion

      • Jun Sakai, Hironori Ishikawa, Hideshi Satoh, Setsuko Yamamoto, Shinichi Kojima, Masaharu Kanaoka

      Pages 33-43

   5. Analysis of Antihypertensive Drugs in the Heart of Animal Models

      • Alberto Lázaro, Julio Gállego-Delgado, Julio I. Osende, Jesús Egido, Fernando Vivanco

      Pages 45-58

   6. A Solubility Optimization Protocol for Two-Dimensional Gel Electrophoresis of Cardiac Tissue

      • Brian A. Stanley, Jennifer E. Van Eyk

      Pages 59-65

   7. A Method for the Effective Depletion of Albumin From Cellular Extracts

      • Brian A. Stanley, Jennifer E. Van Eyk

      Pages 67-70

3. Heart Subproteomes

   1. Front Matter

      Pages 71-71

      PDF

   2. Subcellular Fractionation

      • Mariana Eça, Lukas Alfons Huber

      Pages 73-85

   3. Subfractionation of Heart Tissue

      • Lesley A. Kane, Irina Neverova, Jennifer E. Van Eyk

      Pages 87-90

   4. Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and SELDI-TOF

      • Diane E. Bovenkamp, Brian A. Stanley, Jennifer E. Van Eyk

      Pages 91-102

   5. Proteomic Analysis of the Subunit Composition of Complex I (NADH:Ubiquinone Oxidoreductase) From Bovine Heart Mitochondria

      • Ian M. Fearnley, Joe Carroll, John E. Walker

      Pages 103-125

   6. Identification of Targets of Phosphorylation in Heart Mitochondria

      • Roberta A. Gottlieb

      Pages 127-137

4. Proteomics of Human Atheroma Plaques

   1. Front Matter

      Pages 139-139

      PDF

   2. Characterization of the Human Atheroma Plaque Secretome by Proteomic Analysis

      • Mari-Carmen Durán, Jose L. Martin-Ventura, Sebastián Mas, Maria G. Barderas, Verónica M. Dardé, Ole N. Jensen et al.

      Pages 141-150

   3. Characterization of HSP27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome

      • Mari-Carmen Durán, Elisabetta Boeri-Erba, Shabaz Mohammed, Jose L. Martin-Ventura, Jesús Egido, Fernando Vivanco et al.

      Pages 151-163

   4. Western Array Analysis of Human Atherosclerotic Plaques

      • Wim Martinet

      Pages 165-178

5. The Proteome of Cells of the Cardiovascular System

   1. Front Matter

      Pages 179-179

      PDF

   2. The Proteome of Endothelial Cells

      • Jesús González-Cabrero, Mayte Pozo, Mari-Carmen Durán, Rosario de Nicolás, Jesús Egido, Fernando Vivanco

      Pages 181-198

Over the past few years, the power and potential of proteomics has become widely recognized. The use of proteomics for the study of complex diseases is increasing and is particularly applicable to cardiovascular disease, the leading cause of death in developed countries. The ability to investigate the complete proteome provides a critical tool toward elucidating the complex and multif- torial basis of cardiovascular biology, especially disease processes. Proteomics involves the integration of a number of technologies with the aim of analyzing all the proteins expressed by a biological system in response to various stimuli under different pathophysiological conditions. The proteomic approach offers the ability to evaluate simultaneously the changes in protein expression and cell signaling pathways in response to such conditions as atherosclerosis, c- diac hypertrophy, stroke, or heart failure. Cardiovascular Proteomics: Methods and Protocols covers many of the above aspects of the proteomic approach in the cardiovascular field. This v- ume takes the reader through the complete process of proteomic analysis, from the obtention of specific heart proteins (troponin I) to the new techniques of identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, or the application of phage display techniques to decipher the molecular diversity of blood vessels.

• Proteomics
• Translation
• Vivo
• bioinformatics
• cardiovascular
• hypertension
• platelets
• protein sequence
• proteins

"...a fine collection of methods and protocols currently employed in cardiovascular proteomics that will be of great use to all who are invovled in cardiovascular research." -Proteomics Clin. Appl.

"...a thorough set of protocols for the cardiac biology community.  Most laboratories will have some of these in place, but can profit from the broad coverage of this book." -Doody's Book Review, Weighted Numerical Score: 76 - 3 stars

• Department of Immunology, Fundación Jiménez Díaz, Universidad Complutense, Madrid, Spain

  Fernando Vivanco

• Department of Biochemistry and Molecular Biology I, Universidad Complutense, Madrid, Spain

  Fernando Vivanco

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