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  • © 2007

Macromolecular Crystallography Protocols, Volume 1

Preparation and Crystallization of Macromolecules

Part of the book series: Methods in Molecular Biology (MIMB, volume 363)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-xiii
  2. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag

    • Joseph E. Tropea, Scott Cherry, Sreedevi Nallamsetty, Christophe Bignon, David S. Waugh
    Pages 1-19
  3. Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project

    • Sophie Quevillon-Cheruel, Bruno Collinet, Lionel Trésaugues, Philippe Minard, Gilles Henckes, Robert Aufrère et al.
    Pages 21-37
  4. Protein Engineering

    • Sonia Longhi, François Ferron, Marie-Pierre Egloff
    Pages 59-90
  5. Improving Marginal Crystals

    • Charles W. Carter Jr., Madeleine Riès-Kautt
    Pages 153-174
  6. Crystallization of Protein-DNA Complexes

    • Thomas Hollis
    Pages 225-237
  7. Preparation and Crystallization of RNA

    • Barbara L. Golden
    Pages 239-257
  8. Crystallization of RNA-Protein Complexes

    • Eiji Obayashi, Chris Oubridge, Daniel Pomeranz Krummel, Kiyoshi Nagai
    Pages 259-276
  9. Back Matter

    Pages 277-279

About this book

Macromolecular Crystallography Protocols, now in two volumes, examines major developments that have occurred since publication of the acclaimed first edition nearly a decade ago. Volume 1, Preparation and Crystallization of Macromolecules and Volume 2, Structure Determination, explore recent advances that have accelerated the pace of structural determination and made crystallography accessible to a broader range of investigators.

Volume 1 is composed of detailed protocols for the preparation and optimization of crystals, including tips from the experts on the best methods for inducing proteins to adopt their crystalline form. Volume 2 complements the first volume by addressing laboratory techniques for crystal handling and structural characterization, as well as computational techniques for data collection, phasing, and refinement. The volume concludes with a detailed and insightful survey of available crystallographic software. These volumes will be an indispensable reference for obtaining macromolecular crystals and determining their three-dimensional structure.

Editors and Affiliations

  • Department of Microbiology and Molecular Genetics, University of Vermont, Burlington

    Sylvie Doublié

Bibliographic Information

  • Book Title: Macromolecular Crystallography Protocols, Volume 1

  • Book Subtitle: Preparation and Crystallization of Macromolecules

  • Editors: John M. Walker, Sylvie Doublié

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-209-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-292-6Published: 05 February 2007

  • Softcover ISBN: 978-1-61737-475-3Published: 10 November 2010

  • eBook ISBN: 978-1-59745-209-0Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 280

  • Number of Illustrations: 49 b/w illustrations

  • Topics: Biochemistry, general, Cell Biology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access