Editors:
- Fully updated from the first edition to offer cutting edge methods for manipulation of Mycobacteria
- Includes methods for preparation and analysis of sub-cellular fractions
- Provides description of genetic systems and instructions for manipulating the genome
- Contains techniques for studying gene expression, singly and globally, and updated techniques for mutagenesis and gene essentiality testing
- With detailed protocols for genomic, transcriptomic and proteomic analysis, and new molecular methods for typing and drug susceptibility profiling
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 465)
Buy it now
Buying options
Tax calculation will be finalised at checkout
Other ways to access
This is a preview of subscription content, log in via an institution to check for access.
Table of contents (28 protocols)
-
Front Matter
About this book
Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need. Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field. Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biologyâ„¢ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.
Editors and Affiliations
-
Queen Mary's School of Med. & Dentistry, Inst. Cell & Molecular Science, Barts and the London, London, United Kingdom
Tanya Parish, Amanda Claire Brown
Bibliographic Information
Book Title: Mycobacteria Protocols
Editors: Tanya Parish, Amanda Claire Brown
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-207-6
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-58829-889-8Published: 20 October 2008
Softcover ISBN: 978-1-61737-826-3Published: 26 May 2011
eBook ISBN: 978-1-59745-207-6Published: 18 June 2010
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIX, 456
Additional Information: Originally published as volume 101 in this series
Topics: Bacteriology, Microbiology, Biochemistry, general, Laboratory Medicine