Editors:
- Explores a wide variety of model systems, including human, mouse, sea urchin, starfish, zebrafish, and Xenopus oocytes, transformed Caenorhabditis elegans, and tissue culture cells
- Contains detailed methods easily adaptable to virtually any system
- Covers gene and protein function in living cells, analysis of signal transduction pathways in vivo, single cell analysis, systems biology in a living cell, quantitative analysis of signaling transduction, antisense morpholino oligonucleotide and RNAi analysis, quantitative microinjection, and analysis of Gene Expression, among other topics
- Expands the capabilities of cellular and molecular biology laboratories and provides scientists with a valuable mechanistic understanding
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 518)
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Table of contents (15 protocols)
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Front Matter
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Back Matter
About this book
As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.
Reviews
From the reviews:
"Chapters cover all of the hot topics in which the technique can be used (namely, all the research field you are involved!) and so many different model systems … associated with other master technique (e.g., western blot analysis). … the beginners can immediately get good self-trainings tricks that will ensure them to reach good results. … I would like all the readers … to get a copy of the book: I am sure they will broad the paraphernalia usefull to carry on succesfull researches." (CarloAlberto Redi, European Journal of Histochemistry, October, 2009)
Editors and Affiliations
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Dept. Biological Sciences, Florida Institute of Technology, Melbourne, U.S.A.
David J. Carroll
Bibliographic Information
Book Title: Microinjection
Book Subtitle: Methods and Applications
Editors: David J. Carroll
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-202-1
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-58829-884-3Published: 12 December 2008
Softcover ISBN: 978-1-4939-6090-3Published: 23 August 2016
eBook ISBN: 978-1-59745-202-1Published: 16 December 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 214
Topics: Biotechnology, Cell Culture