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RNAi

Design and Application

  • Book
  • © 2008

Overview

Editors:
  1. Sailen Barik

    1. Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile

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  • Step-by-step instructions in a laboratory bench protocol format
  • Detailed lists of the necessary equipment and reagents
  • Tips on troubleshooting from actual experience of the experts
  • Example data set in most chapters
  • Variety of organisms and applications

Part of the book series: Methods in Molecular Biology (MIMB, volume 442)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Application of RNAi in Diverse Organisms

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Principles of Dicer Substrate (D-siRNA) Design and Function

      • Mohammed Amarzguioui, John J. Rossi
      Pages 3-10

    3. Expression, Purification, and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymes

      • Xuecheng Ye, Qinghua Liu
      Pages 11-27

    4. In vitro RNA Cleavage Assay for Argonaute-Family Proteins

      • Keita Miyoshi, Hiroshi Uejima, Tomoko Nagami-Okada, Haruhiko Siomi, Mikiko C. Siomi
      Pages 29-43

    5. Identifying siRNA-Induced Off-Targets by Microarray Analysis

      • Emily Anderson, Queta Boese, Anastasia Khvorova, Jon Karpilow
      Pages 45-63

  3. Application of RNAi in Diverse Organisms

    1. Front Matter

      Pages 65-65
      Download chapter PDF

    2. Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis

      • Doreen E. Wesche-Soldato, Joanne Lomas-Neira, Mario Perl, Chun-Shiang Chung, Alfred Ayala
      Pages 67-73

    3. Nasal Delivery of siRNA

      • Vira Bitko, Sailen Barik
      Pages 75-82

    4. RNA Interference as a Genetic Tool in Trypanosomes

      • Vivian Bellofatto, Jennifer B. Palenchar
      Pages 83-94

    5. Lentivirus-Mediated RNA Interference in Mammalian Neurons

      • Scott Q. Harper, Pedro Gonzalez-Alegre
      Pages 95-112

    6. Silencing Genes by RNA Interference in the Protozoan Parasite Entamoeba histolytica

      • Carlos F. Solis, Nancy Guillén
      Pages 113-128

    7. Use of RNAi in C. elegans

      • Tsuyoshi Ohkumo, Chikahide Masutani, Toshihiko Eki, Fumio Hanaoka
      Pages 129-137

    8. Application of siRNA Against SARS in the Rhesus Macaque Model

      • Qingquan Tang, Baojian Li, Martin Woodle, Patrick Y. Lu
      Pages 139-158

    9. siRNA and shRNA as Anticancer Agents in a Cervical Cancer Model

      • Wenyi Gu, Lisa Putral, Nigel McMillan
      Pages 159-172

    10. Identification and Expression Analysis of Small RNAs During Development

      • Toshiaki Watanabe, Hiroshi Imai, Naojiro Minami
      Pages 173-185

    11. Screening and Identification of Virus-Encoded RNA Silencing Suppressors

      • Sumona Karjee, Mohammad Nurul Islam, Sunil K. Mukherjee
      Pages 187-203

    12. Application of RNA Interference in Functional Genomics Studies of a Social Insect

      • Michael E. Scharf, Xuguo Zhou, Margaret A. Schwinghammer
      Pages 205-229

    13. Temporal Control of Gene Silencing by in ovo Electroporation

      • Thomas Baeriswyl, Olivier Mauti, Esther T. Stoeckli
      Pages 231-244

    14. Altering Flower Color in Transgenic Plants by RNAi-Mediated Engineering of Flavonoid Biosynthetic Pathway

      • Yoshikazu Tanaka, Noriko Nakamura, Junichi Togami
      Pages 245-257
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Keywords

About this book

RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants.

Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.

Editors and Affiliations

  • Department of Biochemistry and Molecular Biology, University of South Alabama, Mobile

    Sailen Barik

Bibliographic Information

  • Book Title: RNAi

  • Book Subtitle: Design and Application

  • Editors: Sailen Barik

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-191-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-874-4Published: 03 April 2008

  • Softcover ISBN: 978-1-61737-819-5Published: 19 January 2011

  • eBook ISBN: 978-1-59745-191-8Published: 26 March 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 270

  • Number of Illustrations: 45 b/w illustrations, 3 illustrations in colour

  • Topics: Biochemistry, general, Human Genetics, Molecular Medicine, Cell Biology

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