Agrobacterium Protocols

1. Front Matter

   Pages i-xxiii

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2. Root Plants

   1. Front Matter

      Pages 1-1

      PDF

   2. Carrot (Daucus carota L.)

      • Owen Wally, Jayaraj Jayaraman, Zamir K. Punja

      Pages 3-12

   3. Cassava (Manihot esculenta Crantz)

      • Weston Msikita, Uzoma Ihemere, Dimuth Siritunga, Richard T. Sayre

      Pages 13-24

   4. Potato (Solanum tuberosum L.)

      • Steve Millam

      Pages 25-35

   5. Sweet Potato [Ipomoea batatas (L.) Lam.]

      • Guo-qing Song, Ken-ichi Yamaguchi

      Pages 37-44

3. Turf Grasses

   1. Front Matter

      Pages 45-45

      PDF

   2. Bermudagrass (Cynodon spp.)

      • Yaxin Ge, Zeng-Yu Wang

      Pages 47-53

   3. Perennial Ryegrass (Lolium perenne L.)

      • Fredy Altpeter

      Pages 55-64

   4. Switchgrass (Panicum virgatum L.)

      • Mariya N. Somleva

      Pages 65-74

   5. Tall Fescue (Festuca arundinacea Schreb.)

      • Yaxin Ge, Zeng-Yu Wang

      Pages 75-81

   6. Turf Grasses

      • Chip Longo, Colin Lickwar, Qian Hu, Kimberly Nelson-Vasilchik, David Viola, Joel Hague et al.

      Pages 83-95

4. Woody Species

   1. Front Matter

      Pages 97-97

      PDF

   2. American Elm (Ulmus americana)

      • Andrew E. Newhouse, Franziska Schrodt, Charles A. Maynard, William A. Powell

      Pages 99-112

   3. Cork Oak Trees (Quercus suber L.)

      • Rubén Álvarez, Mariano Toribio, Millán Cortizo, Ricardo-Javier Ordás Fernández

      Pages 113-123

   4. Eucalyptus

      • Zenn-Zong Chen, Cheng-Kuen Ho, In-Suk Ahn, Vincent L. Chiang

      Pages 125-134

   5. Pine (Pinus radiata)

      • Jan Grant, Tracy Dale, Pauline Cooper

      Pages 135-141

   6. Poplar (Populus spp.)

      • Richard Meilan, Caiping Ma

      Pages 143-151

   7. Rubber Tree (Hevea brasiliensis Muell. Arg)

      • Perumal Venkatachalam, Radha Jayashree, Karumamkandathil Rekha, Sreedharannair Sushmakumari, Sankaren Sobha, Parukkuttyamma Kumari Jayasree et al.

      Pages 153-164

5. Tropic Plants

   1. Front Matter

      Pages 165-165

      PDF

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

• DNA
• Flora
• algae
• arabidopsis thaliana
• fungi
• genetic engineering
• molecular biology
• saccharomyces cerevisiae

From the reviews of the second edition:

"This is, as the title suggests, a laboratory manual with protocols for using Agrobacterium tumefaciens to transform plant species … . the book will be a valuable addition to the laboratory bookshelf. The protocols … are clearly set out and the notes explain things … . I was also pleased that some chapters mentioned the need to consider regulatory issues regarding disposal of genetically modified materials, something that is often ignored in lab manuals." (Heather Macdonald, SGM – Society for General Microbiology, 2007)

• Center for Plant Transformation, Plant Science Institute, Iowa State Universtiy, Ames

  Kan Wang

• Department of Agronomy, Iowa State Universtiy, Ames

  Kan Wang

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