Cell'Cell Interactions

1. Front Matter

   Pages i-xiii

   PDF

2. Blood-Brain Barrier and Cell-Cell Interactions: Methods for Establishing In Vitro Models of the Blood-Brain Barrier and Transport Measurements

   • Michael Aschner, Vanessa A. Fitsanakis, Ana Paula Marreilha dos Santos, Luisa Olivi, Joseph P. Bressler

   Pages 1-15

3. Interactions of Intestinal Epithelial Cells With Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences

   • Geraldine Canny, Alexander Swidsinski, Beth A. McCormick

   Pages 17-35

4. The Role of Junctional Adhesion Molecules in Interactions Between Vascular Cells

   • Triantafyllos Chavakis, Valeria Orlova

   Pages 37-50

5. Cell-Cell Interactions on Solid Matrices

   • Nancy A. Louis, Dionne Daniels, Sean P. Colgan

   Pages 51-59

6. Cell-Cell Interactions in the Kidney: Inducible Expression of Mutant G Protein α-Subunits in Madin-Darby Canine Kidney Cells for Studies of Epithelial Cell Junction Structure and Function

   • Ernesto Sabath, Bradley M. Denker

   Pages 61-72

7. Nucleotide Metabolism and Cell-Cell Interactions

   • Holger K. Eltzschig, Thomas Weissmüller, Alice Mager, Tobias Eckle

   Pages 73-87

8. Analysis of Mammalian Sperm-Egg Membrane Interactions During In Vitro Fertilization

   • Genevieve B. Wortzman, Allison J. Gardner, Janice P. Evans

   Pages 89-101

9. Collagen Gel Contraction Assay

   • Peter Ngo, Punitha Ramalingam, Jonathan A. Phillips, Glenn T. Furuta

   Pages 103-109

10. Methods to Assess Tissue Permeability

    • Juan C. Ibla, Joseph Khoury

    Pages 111-117

11. Bacterial–Bacterial Cell Interactions in Biofilms: Detection of Polysaccharide Intercellular Adhesins by Blotting and Confocal Microscopy

    • Kimberly K. Jefferson, Nuno Cerca

    Pages 119-126

12. A Biochemical Method for Tracking Cholera Toxin Transport From Plasma Membrane to Golgi and Endoplasmic Reticulum

    • Heidi E. De Luca, Wayne I. Lencer

    Pages 127-139

13. Isolation and Culture of Murine Heart and Lung Endothelial Cells for In Vitro Model Systems

    • Yaw-Chyn Lim, Francis W. Luscinskas

    Pages 141-154

14. Expression Cloning of Signaling Proteins Regulated by Cell Adhesion

    • Michelle L. Matter, Joe W. Ramos

    Pages 155-165

15. Assays for the Calcium Sensitivity of Desmosomes

    • Anita J. Merritt, Anthea Scothern, Tanusree Bhattacharyya

    Pages 167-183

16. Tight Junctions and Cell–Cell Interactions

    • Markus Utech, Matthias Brüwer, Asma Nusrat

    Pages 185-195

17. Simultaneous Immunofluorescence Detection of Cadherin and β-Catenin in Mouse Tissue Sections

    • Nobuko Obara

    Pages 197-204

18. In Vitro Neutrophil Transepithelial Migration

    • Winston Y. Lee, Alex C. Chin, Susan Voss, Charles A. Parkos

    Pages 205-215

19. The Blot Rolling Assay: A Method for Identifying Adhesion Molecules Mediating Binding Under Shear Conditions

    • Robert Sackstein, Robert Fuhlbrigge

    Pages 217-226

20. Cell-Cell Interaction in the Transcellular Biosynthesis of Novel ω-3-Derived Lipid Mediators

    • Nan Chiang, Charles N. Serhan

    Pages 227-250

A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

• bacteria
• biology
• biosynthesis
• cell
• cloning
• membrane
• metabolism
• microscopy
• migration
• molecular biology
• protein
• tissue

• Center for Experimental Therapeutics and Reperfusion Injury, Brigham

  Sean P. Colgan

• Women’s Hospital, Havard Medical School, Boston, USA

  Sean P. Colgan

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