Skip to main content
SpringerLink
Log in

Osteoporosis

Methods and Protocols

  • Book
  • © 2008

Overview

Editors:
  1. Jennifer J. Westendorf

    1. Department of Orthopedic Surgery, Mayo Clinic, Rochester, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Contains simple and complete protocols for culturing osteoblasts and osteoclasts
  • Explains how to utilize various rodent models to study and treat osteoporosis
  • Provides protocols for increasing or decreasing gene expression in bone cells
  • Examines molecular, genetic and imaging techniques to study bone
  • Covers multiple protocols to study mechanical loading on bone and its cells
  • Each chapter contains troubleshooting tips from respected experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 455)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (23 protocols)

  1. Front Matter

    Pages i-xix
    Download chapter PDF

  2. In Vitro Models

    1. Isolation and Culture of Rodent Osteoprogenitor Cells

      • Thomas A. Owen, Lydia C. Pan
      Pages 3-18

    2. Osteoclast Culture and Resorption Assays

      • Elizabeth W. Bradley, Merry Jo Oursler
      Pages 19-35

    3. Assessing New Bone Formation in Neonatal Calvarial Organ Cultures

      • Khalid S. Mohammad, John M. Chirgwin, Theresa A. Guise
      Pages 37-50

    4. Detection of Apoptosis of Bone Cells In Vitro

      • Teresita Bellido, Lilian I. Plotkin
      Pages 51-75

  3. In Vivo Rodent Models

    1. In Vivo Parathyroid Hormone Treatments and RNA Isolation and Analysis

      • Li Xin, Qin Ling, C. Partridge Nicola
      Pages 79-87

    2. Assessment of Bone Formation Capacity Using In vivo Transplantation Assays: Procedure and Tissue Analysis

      • Basem M. Abdallah, Nicholas Ditzel, Moustapha Kassem
      Pages 89-100

    3. Ossicle and Vossicle Implant Model Systems

      • Glenda J. Pettway, Laurie K. McCauley
      Pages 101-110

    4. Utility of the Ovariectomized Rat as a Model for Human Osteoporosis in Drug Discovery

      • Yogendra P. Kharode, Michael C. Sharp, Peter V.N. Bodine
      Pages 111-124

    5. Orchidectomy Models of Osteoporosis

      • Stéphane Blouin, Hélène Libouban, Marie Françoise Moreau, Daniel Chappard
      Pages 125-134

  4. Methods of Gene Delivery

    1. Gene Delivery by Adenoviruses

      • Renny T. Franceschi, Chunxi Ge
      Pages 137-147

    2. Lentivirus Delivery of shRNA Constructs into Osteoblasts

      • Marc N. Wein, Dallas C. Jones, Laurie H. Glimcher
      Pages 149-155

    3. Gene Delivery by Retroviruses

      • Valerie Deregowski, Ernesto Canalis
      Pages 157-162

  5. Molecular and Genetic Techniques

    1. Chromatin Immunoprecipitation Assays: Application of ChIP-on-Chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells

      • Margaretha van der Deen, Mohammad Q. Hassan, Jitesh Pratap, Nadiya M. Teplyuk, Daniel W. Young, Amjad Javed et al.
      Pages 165-176

    2. Identification of Transcription Factor Target Genes by ChIP Display

      • Artem Barski, Steven Pregizer, Baruch Frenkel
      Pages 177-190

    3. Application of the Laser Capture Microdissection Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo

      • Dafna Benayahu, Rina Socher, Irena Shur
      Pages 191-201

    4. Quantitative Trait Loci Mapping

      • Dong-Hai Xiong, Jian-Feng Liu, Yan-Fang Guo, Yan Guo, Tie-Lin Yang, Hui Jiang et al.
      Pages 203-235

  6. Imaging Techniques

    1. In Situ Nuclear Organization of Regulatory Machinery

      • Shirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali et al.
      Pages 239-259

    2. Bioluminescent Imaging in Bone

      • Yoram Zilberman, Yossi Gafni, Gadi Pelled, Zulma Gazit, Dan Gazit
      Pages 261-272

    3. Micro-Computed Tomography: A Method for the Non-Destructive Evaluation of the Three-Dimensional Structure of Biological Specimens

      • Martin Stauber, Ralph Müller
      Pages 273-292
Back to top

Keywords

About this book

In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. The topics covered in this volume include in vitro models, in vivo models utilized for drug testing, tissue engineering and osteoporosis studies in either gender, state-of-the-art molecular techniques to assess single genes or for global genomic analysis, powerful imaging techniques, and many more. As a volume in the highly successful Methods in Molecular Biology™ series, each chapter provides a brief introduction, a list of necessary materials, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Osteoporosis: Methods and Protocols is the perfect guide for both new and experienced experimentalists attempting to study the devastating silent disease known as osteoporosis.

Editors and Affiliations

  • Department of Orthopedic Surgery, Mayo Clinic, Rochester, USA

    Jennifer J. Westendorf

Bibliographic Information

  • Book Title: Osteoporosis

  • Book Subtitle: Methods and Protocols

  • Editors: Jennifer J. Westendorf

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-104-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-828-7Published: 16 April 2008

  • Softcover ISBN: 978-1-62703-847-8Published: 03 November 2014

  • eBook ISBN: 978-1-59745-104-8Published: 08 May 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIX, 354

  • Topics: Orthopedics, Molecular Medicine, Cell Biology, Imaging / Radiology

Publish with us

Policies and ethics

Back to top

Search

Navigation