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  • © 2008

Plant Virology Protocols

From Viral Sequence to Protein Function

  • Offers step by step reproducible laboratory protocols written by experts in the field of plant sciences, virology, and genomics
  • Focus on the novel and emerging techniques used to analyze, understand, and treat plant viruses
  • Covers the spectrum of plant virology protocols, from general introductions to typical plant viral proteins, techniques for investigating viral acid sequences, through the emerging area of genomics and hist factor interactions
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 451)

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Table of contents (44 protocols)

  1. Front Matter

    Pages i-xx
  2. General Introduction

    1. Plant–Virus Interactions

      • Peter Palukaitis, John P. Carr, James E. Schoelz
      Pages 3-19
    2. Role of Capsid Proteins

      • John F. Bol
      Pages 21-31
    3. Role of Plant Virus Movement Proteins

      • Michael Taliansky, Lesley Torrance, Natalia O. Kalinina
      Pages 33-54
    4. Role of Silencing Suppressor Proteins

      • József Burgyán
      Pages 69-79
    5. Role of Vector-Transmission Proteins

      • Véronique Ziegler-Graff, Véronique Brault
      Pages 81-96
  3. Viral Nucleic Acid Sequence/Function Analysis

    1. In Vivo Analyses of Viral RNA Translation

      • William R. Staplin, W. Allen Miller
      Pages 99-112
    2. In Vitro Analysis of Translation Enhancers

      • Aurélie M. Rakotondrafara, W. Allen Miller
      Pages 113-124
    3. Identification of Plant Virus IRES

      • Sek-Man Wong, Dora Chin-Yen Koh, Dingxiang Liu
      Pages 125-133
    4. Begomoviruses: Molecular Cloning and Identification of Replication Origin

      • Lilian H. Florentino, Anésia A. Santos, Francisco M. Zerbini, Elizabeth P. B. Fontes
      Pages 145-166
    5. Analysis of Viroid Replication

      • Ricardo Flores, MaríDa-Eugenia Gas, Diego Molina, Carmen Hernández, José-Antonio Darós
      Pages 167-183
    6. In Situ Detection of Plant Viruses and Virus-Specific Products

      • Andrew J. Maule, Zoltán Havelda
      Pages 201-216
    7. Detection of siRNAs and miRNAs

      • Sakari Kauppinen, Zoltán Havelda
      Pages 217-227
    8. Cloning of Short Interfering RNAs from Virus-Infected Plants

      • Thien X. Ho, Rachel Rusholme, Tamas Dalmay, Hui Wang
      Pages 229-241
    9. Solution Structure Probing of RNA Structures

      • Marc R. Fabian, K. Andrew White
      Pages 243-250
    10. RNA Encapsidation Assay

      • Padmanaban Annamalai, A.L.N. Rao
      Pages 251-264
  4. Protein Analysis and Investigation of Protein Function

About this book

Following the considerable success of the first edition of Plant Virology Protocols, this exciting new edition covers the many new techniques that are now applied to the examination and understanding of plant viruses. Each section of Plant Virology Protocols, Second Edition presents the most novel methods and protocols to allow researchers more effective approaches to study plant viruses. The many subjects covered in this important new edition range from techniques for investigating viral nucleic acid sequence, protein analysis and investigation of protein function, techniques for microscopy/GFP visualization and analysis/protein tagging/generation of infectious clones and other such tools, as well as areas of genomics and interactions with host factors and plant-based studies. Plant Virology Protocols, Second Edition: From viral sequence to protein function is indispensable to laboratory investigators studying plant viruses.

Reviews

From the reviews of the second edition:

"This book describes a wide range of techniques on the molecular analyses of plant viruses and some of their interactions with their hosts. Many techniques could also be of interest to scientists studying animal and bacterial viruses. … this book should be of great use to virology researchers who take the correct approach." (Roger Hull, Microbiology Today, February, 2009)

Editors and Affiliations

  • University of Bristol, Bristol, UK

    Gary D. Foster

  • University of Aarhus, Aarhus, Denmark

    I. Elisabeth Johansen

  • University of Warwick, Warwick, UK

    Yiguo Hong

  • University of Kentucky, Kentucky, USA

    Peter D. Nagy

Bibliographic Information

  • Book Title: Plant Virology Protocols

  • Book Subtitle: From Viral Sequence to Protein Function

  • Editors: Gary D. Foster, I. Elisabeth Johansen, Yiguo Hong, Peter D. Nagy

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-102-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2008

  • Hardcover ISBN: 978-1-58829-827-0Published: 07 March 2008

  • Softcover ISBN: 978-1-61737-794-5Published: 10 December 2010

  • eBook ISBN: 978-1-59745-102-4Published: 16 March 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XX, 677

  • Number of Illustrations: 61 b/w illustrations, 1 illustrations in colour

  • Topics: Plant Sciences, Virology, Plant Genetics and Genomics

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access