Ion Channels

1. Front Matter

   Pages i-xii

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2. Methods for Exogenous Expression of Ion Channels in Cells

   1. Front Matter

      Pages 1-38

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   2. Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System

      • Alexander Staruschenko, Rachell E. Booth, Oleh Pochynyuk, James D. Stockand, Qiusheng Tong

      Pages 3-13

   3. Overexpression of Proteins in Neurons Using Replication-Deficient Virus

      • Richard M. Ahlquist, Jane M. Sullivan

      Pages 15-26

   4. Exogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery System

      • Nikita Gamper, Mark S. Shapiro

      Pages 27-38

3. Methods for Studying Channel Structure-function

   1. Front Matter

      Pages 39-77

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   2. Tertiary and Quaternary Structure Formation of Voltage-Gated Potassium Channels

      • John M. Robinson, Andrey Kosolapov, Carol Deutsch

      Pages 41-52

   3. Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte Expression System

      • Farhad Kosari, Shaohu Sheng, Thomas R. Kleyman

      Pages 53-63

   4. Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis

      • Jie Zheng

      Pages 65-77

4. Methods for Studying Channel Regulation and Physiological Function

   1. Front Matter

      Pages 79-153

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   2. Probing the Effects of Phosphoinositides on Ion Channels

      • Chou-Long Huang

      Pages 81-87

   3. Epithelial Sodium Channel in Planar Lipid Bilayers

      • Bakhrom K. Berdiev, Dale J. Benos

      Pages 89-99

   4. A Simple In Vivo Method for Assessing Changes of Membrane-Bound Ion Channel Density in Xenopus Oocytes

      • Mouhamed S. Awayda, Weijian Shao, Ivana Vukojicic, Abderrahmane Bengrine

      Pages 101-115

   5. Preparation of Cortical Brain Slices for Electrophysiological Recording

      • Costa M. Colbert

      Pages 117-125

   6. Juxtacellular Labeling and Chemical Phenotyping of Extracellularly Recorded Neurons In Vivo

      • Glenn M. Toney, Lynette C. Daws

      Pages 127-137

   7. Carbon Fiber Amperometry in the Study of Ion Channels and Secretion

      • Duk-Su Koh

      Pages 139-153

5. Methods for Studying Channelopathies, Genetic Screening, and Molecular Biology

   1. Front Matter

      Pages 155-205

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   2. Genetic Screening for Functionality of Bacterial Potassium Channel Mutants Using K⁺ Uptake-Deficient Escherichia coli

      • Lyubov V. Parfenova, Brad S. Rothberg

      Pages 157-165

   3. KCNQ1 K+ Channel—Mediated Cardiac Channelopathies

      • Gildas Loussouarn, Isabelle Baró, Denis Escande

      Pages 167-183

   4. Tissue-Specific Transgenic and Knockout Mice

      • Gildas Loussouarn, Isabelle Baró, Denis Escande

      Pages 185-205

Internationally recognized biomedical scientists describe recent technological breakthroughs and demonstrate their use in successful experimental designs. The diverse applications range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, for studying channel regulation and physiological function, and for genetic screening and investigating channelopathies.

• Escherichia coli
• Lipid
• Vivo
• molecular biology
• quaternary structure
• regulation

• University of Texas Health Science Center, San Antonio, USA

  James D. Stockand, Mark S. Shapiro

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