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Microchip Capillary Electrophoresis

Methods and Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Charles S. Henry

    1. Colorado State University, Fort Collins, USA

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 339)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Microchip Capillary Electrophoresis

    1. Microchip Capillary Electrophoresis

      • Charles S. Henry
      Pages 1-9

  3. Microchip Fabrication Methods

    1. Front Matter

      Pages 11-46
      Download chapter PDF

    2. Fabrication of a Glass Capillary Electrophoresis Microchip With Integrated Electrodes

      • Mark M. Crain, Robert S. Keynton, Kevin M. Walsh, Thomas J. Roussel Jr., Richard P. Baldwin, John F. Naber et al.
      Pages 13-26

    3. Micro-Molding for Poly(dimethylsiloxane) Microchips

      • Carlos D. García, Charles S. Henry
      Pages 27-35

    4. Fabrication of Polymer Microfluidic Systems by Hot Embossing and Laser Ablation

      • Laurie E. Locascio, David J. Ross, Peter B. Howell, Michael Gaitan
      Pages 37-46

  4. Surface Modification Methods

    1. Front Matter

      Pages 47-64
      Download chapter PDF

    2. Surface Modification Methods for Enhanced Device Efficacy and Function

      • Barbara J. Jones, Mark A. Hayes
      Pages 49-56

    3. Polyelectrolyte Coatings for Microchip Capillary Electrophoresis

      • Yan Liu, Charles S. Henry
      Pages 57-64

  5. Detection Methods for Microchip Capillary Electrophoresis

    1. Front Matter

      Pages 65-126
      Download chapter PDF

    2. Interfacing Microchip Capillary Electrophoresis With Electrospray Ionization Mass Spectrometry

      • Trust Razunguzwa, Aaron T. Timperman
      Pages 67-83

    3. Interfacing Amperometric Detection With Microchip Capillary Electrophoresis

      • R. Scott Martin
      Pages 85-112

    4. Conductivity Detection on Microchips

      • Roland Hergenröder, Benedikt Graβ
      Pages 113-126

  6. Applications of Microchip Capillary Electrophoresis

    1. Front Matter

      Pages 127-231
      Download chapter PDF

    2. DNA Separations

      • Andrea W. Chow
      Pages 129-143

    3. Protein Separations

      • Andrea W. Chow
      Pages 145-158

    4. Microchip Capillary Electrophoresis

      • Barbara A. Fogarty, Nathan A. Lacher, Susan M. Lunte
      Pages 159-186

    5. Measuring Electroosmotic Flow in Microchips and Capillaries

      • S. Douglass Gilman, Peter J. Chapman
      Pages 187-201

    6. Single Cell Analysis on Microfluidic Devices

      • Christopher T. Culbertson
      Pages 203-216

    7. Rapid DNA Amplification in Glass Microdevices

      • Christopher J Easley, Lindsay A. Legendre, James P. Landers, Jerome P. Ferrance
      Pages 217-231
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Keywords

About this book

Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.

Reviews

"From this book the reader can acquire a basic command of the technique relatively fast and, if required, can move on to in-depth fundamental, or specific information in the literature of this explosively growing area of science." -Molecular Biotechnology

Editors and Affiliations

  • Colorado State University, Fort Collins, USA

    Charles S. Henry

Bibliographic Information

  • Book Title: Microchip Capillary Electrophoresis

  • Book Subtitle: Methods and Protocols

  • Editors: Charles S. Henry

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597450766

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-293-3Published: 01 March 2006

  • Softcover ISBN: 978-1-61737-476-0Published: 10 November 2010

  • eBook ISBN: 978-1-59745-076-8Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 238

  • Topics: Analytical Chemistry

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