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  • © 2009

Plant Pathology

Techniques and Protocols

Editors:

  • Provides researchers with innovative methodologies for plant pathogen diagnostics
  • Written by experts from internationally renowned institutions
  • Cross-cutting approach to diagnostics, including molecular, serological, and traditional methodologies
  • All classes of plant pathogen covered in methodologies
  • Provides methods for crop plant genetic validation
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 508)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-ix
  2. Bioassay for Diagnosis of Plant Viruses

    • Isla A. Browning
    Pages 1-13
  3. Preparation of Immunogens and Production of Antibodies

    • Hei-ti Hsu, Tsung-chi Chen, Chin-an Chang, Shyi-dong Yeh
    Pages 37-50
  4. Production of Monoclonal Antibodies to Plant Pathogens

    • Christopher R. Thornton
    Pages 63-74
  5. Detection of Four Major Bacterial Potato Pathogens

    • Ellen M. Kerr, Greig Cahill, Karen Fraser
    Pages 101-114
  6. Erwinia amylovora: Modern Methods for Detection and Differentiation

    • Antonet M. Svircev, Won-Sik Kim, Susan M. Lehman, Alan J. Castle
    Pages 115-129
  7. Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani

    • Nikki D. Charlton, Stellos M. Tavantzis, Marc A. Cubeta
    Pages 171-182
  8. Immunocapture-PCR for Plant Virus Detection

    • Vincent Mulholland
    Pages 183-192
  9. Multiplex Polymerase Chain Reaction (PCR) and Real-time Multiplex PCR for the Simultaneous Detection of Plant Viruses

    • V. Pallas, J. Sanchez-Navarro, A. Varga, F. Aparicio, D. James
    Pages 193-208
  10. Fluorescent-based Techniques for Viral Detection, Quantification andCharacterisation

    • Mathieu Rolland, Agnès Delaunay, Emmanuel Jacquot
    Pages 209-234
  11. Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR

    • Neil Boonham, Lynn Laurenson, Rebecca Weekes, Rick Mumford
    Pages 249-258

About this book

Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Composed in the highly successful Methods in Molecular Biologyâ„¢ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will soon prove to be indispensable, both to novices and expert researchers alike.

Reviews

From the reviews:

"Plant Pathology: Techniques and Protocols provides a collection of methods for the diagnosis and detection of plant pathogens. … the book can also be used to troubleshoot particular techniques and adapt it to any organism of interest. The notes at the end of each chapter are of great utility … . It is a highly practical book and both students and researchers interested or working on detection and diagnosis will find it very useful." (Arantza Rico, Microbiology Today, November, 2009)

Bibliographic Information

  • Book Title: Plant Pathology

  • Book Subtitle: Techniques and Protocols

  • Editors: Robert Burns

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-062-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2009

  • Hardcover ISBN: 978-1-58829-799-0Published: 12 December 2008

  • Softcover ISBN: 978-1-61737-785-3Published: 19 November 2010

  • eBook ISBN: 978-1-59745-062-1Published: 21 April 2009

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 326

  • Number of Illustrations: 42 b/w illustrations

  • Topics: Plant Sciences, Plant Pathology, Virology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access